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- W2485677799 abstract "In order to study the mechanism of the irre- versible effects of BrdUrd on the differentiation of limb bud mesenchyme to cartilage, the reannealing behavior of DNA obtained from such cells was examined. Cells incubated with (3lHlthymidine ((3H)dThd) during days 1 and 2 of culture in- corporated label into repetitive, moderately repetitive, and unique classes of DNA. In contrast, when 5-bromo-2'- (3H)deoxvuridine ((3H)BrdUrd) was added during the first 48 hr (ir sence of 32 ,gM BrdUrd), the label was preferen- tially incorporated into a late moderately repetitive region. Simultaneous incubation of unlabeled BrdUrd and (3HIdThd revealed a selective inhibition of (3H)dThd incorporation into moderately repetitive regions. Cultures incubated dur- ing days 3 and 4 with (3H)dThd incorporated label into all three classes of DNA; however, when (3H)dThd was present during days 3 and 4 in cultures previously incubated with BrdUrd during days 1 and 2, the (3HIdThd was incorporated preferentially in the late moderately repetitive region. The melting behavior of this reannealed DNA was identical with that of the reannealed 1-2 day (3H)BrdUrd-labeled, late mod- erately repetitive DNA. Turnover experiments revealed that whereas there was no loss of (3H)deoxycytidine or (3H)dThd, 37% of (3H)BrdUrd activity was lost from the DNA in 2 days after removal of the isotopes." @default.
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- W2485677799 date "2016-01-01" @default.
- W2485677799 modified "2023-09-27" @default.
- W2485677799 title "Distribution of 5-bromodeoxyuridine and thymidine in the DNA of" @default.
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