FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2487146725> ?p ?o ?g. }

• W2487146725 abstract "As the precursor to metastatic cancer, circulating tumor cells (CTCs) hold tremendous potential for increasing our understanding of tumor metastasis, advancing personalized therapeutics, and have emerged as a significant source of genetic material for clinical guidance. Recent technical advances have enabled the enrichment and genomic characterization of CTCs at the single cell level and have revealed significant and clinically relevant heterogeneity in these rare cells. However, data is lacking for existing methods to link the functional differences of single CTCs to their underlying genomic structure and activity. We have developed an approach which integrates key aspects of biologically based experimentation at the single cell level onto an integrated fluidic circuit. This device allows for the selection and isolation of single CTCs in a size-independent fashion from a pre-enriched population based on fluorescent markers where they can be individually cultured and exposed to a variety of drugs/reagents under environmentally controlled conditions or processed directly for mRNA-seq. If desired, an extracellular matrix can be deposited into each of the single cell culture chambers. Cell activity and phenotype can be monitored in-situ in response to treatment conditions, followed by preparation of the mRNA transcriptome for RNA-seq analysis. As the entire workflow following pre-enrichment is integrated onto a single microfluidic chip, very little manipulation of the single cell is required, serving to preserve the mRNA signature of each cell. To optimize and validate the workflow, PC3 cell lines were spiked-in to blood from healthy donors followed by pre-enrichment for metastatic cancer cells using commercially available CTC isolation platforms. Our microfluidic device further purified and isolated the desired single PC3 cells, and prepared the cells for expression analysis via RNA-seq. The sequencing results further validated the ability of our workflow to isolate rare metastatic cancer cells from human blood and moreover confirmed highly correlated gene expression profiles as compared to cultured PC3 population controls (r = 0.89 across 23,562 genes) and non spike-in single cell samples (r = 0.90 across 23,562 genes). We applied this workflow to the blood of metastatic castration resistant prostate cancer (mCRPC) patients and obtained single cell cDNA from the isolated CTCs from which we were able to generate sequencing libraries. Future work using this system will help establish a robust methodology to enable more complex perturbation and functional studies of single cells from clinical samples. Citation Format: Lukasz Szpankowski, Gayatri Premasekharan, Naveen Ramalingam, Chad Sanada, Anne Leyrat, Brian Fowler, Matthew Edwards, Cassandra Greene, Ilona Holcomb, Charles J. Ryan, Pamela L. Paris, Jay A.A. West. Isolation and mRNA-seq analysis of single CTCs from blood samples using an integrated fluidic circuit for functional single cell studies. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr LB-327." @default.
• W2487146725 created "2016-08-23" @default.
• W2487146725 creator A5001763530 @default.
• W2487146725 creator A5005909565 @default.
• W2487146725 creator A5011237350 @default.
• W2487146725 creator A5011621241 @default.
• W2487146725 creator A5012804612 @default.
• W2487146725 creator A5024057870 @default.
• W2487146725 creator A5028656179 @default.
• W2487146725 creator A5036726954 @default.
• W2487146725 creator A5055816581 @default.
• W2487146725 creator A5077316309 @default.
• W2487146725 creator A5077950247 @default.
• W2487146725 creator A5079091432 @default.
• W2487146725 date "2016-07-15" @default.
• W2487146725 modified "2023-09-26" @default.
• W2487146725 title "Abstract LB-327: Isolation and mRNA-seq analysis of single CTCs from blood samples using an integrated fluidic circuit for functional single cell studies" @default.
• W2487146725 doi "https://doi.org/10.1158/1538-7445.am2016-lb-327" @default.
• W2487146725 hasPublicationYear "2016" @default.
• W2487146725 type Work @default.
• W2487146725 sameAs 2487146725 @default.
• W2487146725 citedByCount "0" @default.
• W2487146725 crossrefType "proceedings-article" @default.
• W2487146725 hasAuthorship W2487146725A5001763530 @default.
• W2487146725 hasAuthorship W2487146725A5005909565 @default.
• W2487146725 hasAuthorship W2487146725A5011237350 @default.
• W2487146725 hasAuthorship W2487146725A5011621241 @default.
• W2487146725 hasAuthorship W2487146725A5012804612 @default.
• W2487146725 hasAuthorship W2487146725A5024057870 @default.
• W2487146725 hasAuthorship W2487146725A5028656179 @default.
• W2487146725 hasAuthorship W2487146725A5036726954 @default.
• W2487146725 hasAuthorship W2487146725A5055816581 @default.
• W2487146725 hasAuthorship W2487146725A5077316309 @default.
• W2487146725 hasAuthorship W2487146725A5077950247 @default.
• W2487146725 hasAuthorship W2487146725A5079091432 @default.
• W2487146725 hasConcept C121608353 @default.
• W2487146725 hasConcept C1491633281 @default.
• W2487146725 hasConcept C185592680 @default.
• W2487146725 hasConcept C2776950831 @default.
• W2487146725 hasConcept C2779013556 @default.
• W2487146725 hasConcept C2908647359 @default.
• W2487146725 hasConcept C502942594 @default.
• W2487146725 hasConcept C54355233 @default.
• W2487146725 hasConcept C61238886 @default.
• W2487146725 hasConcept C70721500 @default.
• W2487146725 hasConcept C71924100 @default.
• W2487146725 hasConcept C81885089 @default.
• W2487146725 hasConcept C86803240 @default.
• W2487146725 hasConcept C99454951 @default.
• W2487146725 hasConceptScore W2487146725C121608353 @default.
• W2487146725 hasConceptScore W2487146725C1491633281 @default.
• W2487146725 hasConceptScore W2487146725C185592680 @default.
• W2487146725 hasConceptScore W2487146725C2776950831 @default.
• W2487146725 hasConceptScore W2487146725C2779013556 @default.
• W2487146725 hasConceptScore W2487146725C2908647359 @default.
• W2487146725 hasConceptScore W2487146725C502942594 @default.
• W2487146725 hasConceptScore W2487146725C54355233 @default.
• W2487146725 hasConceptScore W2487146725C61238886 @default.
• W2487146725 hasConceptScore W2487146725C70721500 @default.
• W2487146725 hasConceptScore W2487146725C71924100 @default.
• W2487146725 hasConceptScore W2487146725C81885089 @default.
• W2487146725 hasConceptScore W2487146725C86803240 @default.
• W2487146725 hasConceptScore W2487146725C99454951 @default.
• W2487146725 hasLocation W24871467251 @default.
• W2487146725 hasOpenAccess W2487146725 @default.
• W2487146725 hasPrimaryLocation W24871467251 @default.
• W2487146725 hasRelatedWork W1994447373 @default.
• W2487146725 hasRelatedWork W2019185202 @default.
• W2487146725 hasRelatedWork W2040556161 @default.
• W2487146725 hasRelatedWork W2485350550 @default.
• W2487146725 hasRelatedWork W2489539696 @default.
• W2487146725 hasRelatedWork W2503401748 @default.
• W2487146725 hasRelatedWork W2535452161 @default.
• W2487146725 hasRelatedWork W2563410844 @default.
• W2487146725 hasRelatedWork W2739694396 @default.
• W2487146725 hasRelatedWork W2742093360 @default.
• W2487146725 hasRelatedWork W2768975823 @default.
• W2487146725 hasRelatedWork W2792939991 @default.
• W2487146725 hasRelatedWork W2798782629 @default.
• W2487146725 hasRelatedWork W2898927445 @default.
• W2487146725 hasRelatedWork W2919358315 @default.
• W2487146725 hasRelatedWork W2955262605 @default.
• W2487146725 hasRelatedWork W2969354027 @default.
• W2487146725 hasRelatedWork W3016204557 @default.
• W2487146725 hasRelatedWork W3017448445 @default.
• W2487146725 hasRelatedWork W3083257483 @default.
• W2487146725 isParatext "false" @default.
• W2487146725 isRetracted "false" @default.
• W2487146725 magId "2487146725" @default.
• W2487146725 workType "article" @default.