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- W2487661579 abstract "Publisher Summary This chapter describes the preparation of primary cultures of somatic plant cells. Primary cultures of somatic plant cells have been established from virtually every organ or tissue and from diverse plants, from the moss to the monocotyledonous angiosperm. Usually, the culture is initiated on an agar medium, with recultures being carried out either on agar or in liquid. Sometimes the culture is started directly in liquid nutrient. Unlike animal cell cultures, where often distinguishing characteristics of the progenitor tissue or organ are retained, plant cultures generally result in the development of a callus, the constituent cells of which rarely manifest characteristics identifiable with the original organ or tissue. In initiating callus cultures of a new plant, it is helpful to consider certain characteristics in regard to (1) the explant, (2) the physical qualities of the nutrient medium, (3) the chemical composition of the medium, and (4) the temperature and light requirements. Explants from mature tissues and organs are expected to be disproportionately high in content of highly differentiated and endopolyploid cells." @default.
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- W2487661579 date "1973-01-01" @default.
- W2487661579 modified "2023-09-27" @default.
- W2487661579 title "Somatic Plant Cells" @default.
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- W2487661579 doi "https://doi.org/10.1016/b978-0-12-427150-0.50047-4" @default.
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