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• W2487974998 abstract "ii TABLE OF CONTENTS iii LIST OF FIGURES v LIST OF TABLES v INTRODUCTION 1 AIMS AND SIGNIFICANCE 5 Conformation and dynamics differences between the GFOGER and GAOGER collagen model peptides 5 Development of a recombinant collagen model peptide for NMR experiments 5 MATERIALS AND METHODS 6 NMR and CD Sample Preperation 6 NMR spectroscopy 6 Two Dimensional HSQCs 7 15 N spin relaxation experiments 7 R1 experiments 7 R2 experiments 8 NOE experiments 9 Hydrogen Exchange experiments 10 Circular Dichroism 12 Bacterial collagen expression and purification 13 Enzyme-Linked Immunosorbent Assay 15 TEV Protease expression and purification 16 Cleavage of the V-TEV-GFPGER 17 RESULTS 18 Secondary structure of integrin binding collagen model peptides 18 Conformational dynamics of integrin binding collagen model peptides measured by NMR 21 Expression of recombinant collagen model peptide 26 DISCUSSION 32 Integrin binding collagen model peptide secondary structure characterization 32" @default.
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• W2487974998 date "2015-01-01" @default.
• W2487974998 modified "2023-09-23" @default.
• W2487974998 title "RELATION OF CONFORMATIONAL AND DYNAMIC DIFFERENCES IN COLLAGEN SEQUENCES TO ALPHA2I DOMAIN INTEGRIN BINDING AFFINITY" @default.
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• W2487974998 doi "https://doi.org/10.7282/t37d2x0b" @default.
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