Matches in SemOpenAlex for { <https://semopenalex.org/work/W2488045658> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2488045658 abstract "Basic Helix-Loop-Helix proteins are transcriptional regulators crucial for many development processes. Using gain- and loss-of-function analysis in zebrafish, the functional role of two members of this protein family, lyl1 (Lymphoblastic leukaemia 1) and Id4 (Inhibitor of differentiation 4) in stem cell fate was determined. Ectopic overexpression of lyl1 resulted in the expansion of haematopoietic stem cell pool and its progeny promoting erythrocyte differentiation and suppressing myeloid differentiation. TALEN-mediated lyl1-/¬- embryos developed normally but displayed distinct marker gene expression during primitive and definitive haematopoiesis establishing a role for lyl1 in both waves of haematopoiesis. During primitive haematopoiesis expression of scl/tal1 and gata1 was unaltered but expression of pu.1 was increased suggesting that lyl1 antagonises myeloid differentiation. Lyl1-deficiency resulted in reduction of Gfi1aa expression during primitive and definitive haematopoiesis. In addition, a reduction in the expression of c-myb in the caudal hematopoietic tissue and rag1 in the thymus was observed indicating that lyl1 is required to maintain the definitive haematopoietic stem cell pool and to drive T lymphopoiesis. In adult zebrafish lyl1 regulates lineage choice driving lymphopoiesis and suppressing myelopoiesis. Morpholino-mediated knockdown of Id4 alone or in combination with p53 resulted in reduced cell proliferation, increased cell death and premature neuronal differentiation. Phenotypic analysis of TALEN-mediated Id4 mutants confirmed that Id4 plays a crucial role in the expansion of neural stem cells and timing of neuronal differentiation. Inhibition of p38MAPK in Id4 morphants as well as Id4-/- mutants resulted in a phenotypic rescue establishing that Id4 negatively regulates p38MAPK activity to ensure normal neurogenesis." @default.
- W2488045658 created "2016-08-23" @default.
- W2488045658 creator A5036902262 @default.
- W2488045658 date "2016-07-17" @default.
- W2488045658 modified "2023-09-27" @default.
- W2488045658 title "TALEN-mediated site-directed mutagenesis of HLH proteins lyl1 and Id4 to reveal their role in haematopoietic and neural stem cell fate" @default.
- W2488045658 hasPublicationYear "2016" @default.
- W2488045658 type Work @default.
- W2488045658 sameAs 2488045658 @default.
- W2488045658 citedByCount "0" @default.
- W2488045658 crossrefType "dissertation" @default.
- W2488045658 hasAuthorship W2488045658A5036902262 @default.
- W2488045658 hasConcept C104317684 @default.
- W2488045658 hasConcept C109159458 @default.
- W2488045658 hasConcept C136571751 @default.
- W2488045658 hasConcept C148738053 @default.
- W2488045658 hasConcept C2776878037 @default.
- W2488045658 hasConcept C28328180 @default.
- W2488045658 hasConcept C502942594 @default.
- W2488045658 hasConcept C54355233 @default.
- W2488045658 hasConcept C79904392 @default.
- W2488045658 hasConcept C81885089 @default.
- W2488045658 hasConcept C86803240 @default.
- W2488045658 hasConcept C95444343 @default.
- W2488045658 hasConcept C95862172 @default.
- W2488045658 hasConcept C97037327 @default.
- W2488045658 hasConceptScore W2488045658C104317684 @default.
- W2488045658 hasConceptScore W2488045658C109159458 @default.
- W2488045658 hasConceptScore W2488045658C136571751 @default.
- W2488045658 hasConceptScore W2488045658C148738053 @default.
- W2488045658 hasConceptScore W2488045658C2776878037 @default.
- W2488045658 hasConceptScore W2488045658C28328180 @default.
- W2488045658 hasConceptScore W2488045658C502942594 @default.
- W2488045658 hasConceptScore W2488045658C54355233 @default.
- W2488045658 hasConceptScore W2488045658C79904392 @default.
- W2488045658 hasConceptScore W2488045658C81885089 @default.
- W2488045658 hasConceptScore W2488045658C86803240 @default.
- W2488045658 hasConceptScore W2488045658C95444343 @default.
- W2488045658 hasConceptScore W2488045658C95862172 @default.
- W2488045658 hasConceptScore W2488045658C97037327 @default.
- W2488045658 hasLocation W24880456581 @default.
- W2488045658 hasOpenAccess W2488045658 @default.
- W2488045658 hasPrimaryLocation W24880456581 @default.
- W2488045658 hasRelatedWork W1660819588 @default.
- W2488045658 hasRelatedWork W1978187353 @default.
- W2488045658 hasRelatedWork W1990184382 @default.
- W2488045658 hasRelatedWork W1997998180 @default.
- W2488045658 hasRelatedWork W2045581957 @default.
- W2488045658 hasRelatedWork W2078311638 @default.
- W2488045658 hasRelatedWork W2086664962 @default.
- W2488045658 hasRelatedWork W2090035935 @default.
- W2488045658 hasRelatedWork W2116024851 @default.
- W2488045658 hasRelatedWork W2139805763 @default.
- W2488045658 hasRelatedWork W2154589633 @default.
- W2488045658 hasRelatedWork W2240718033 @default.
- W2488045658 hasRelatedWork W2460544942 @default.
- W2488045658 hasRelatedWork W2501364036 @default.
- W2488045658 hasRelatedWork W2530170904 @default.
- W2488045658 hasRelatedWork W2548217852 @default.
- W2488045658 hasRelatedWork W2564301342 @default.
- W2488045658 hasRelatedWork W3095933316 @default.
- W2488045658 hasRelatedWork W3185382935 @default.
- W2488045658 hasRelatedWork W766893721 @default.
- W2488045658 isParatext "false" @default.
- W2488045658 isRetracted "false" @default.
- W2488045658 magId "2488045658" @default.
- W2488045658 workType "dissertation" @default.