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- W2488202112 abstract "Abstract A simple method to quantitate protein in the spots of two-dimensional polyacrylamide gel electrophoretograms is described. The system is composed of three different steps:(1) O'Farrell's two-dimensional gel electrophoresis of proteins to be analyzed, (2) staining of the gels with Commassie brilliant blue, and (3) determination of the area and the integrated density of the stained spots by Joyce-Loebl Magiscan-I Image Analysis System. The method can be used for the determination of protein in the range of 0.5 to 100μg/cm2 and the amount of protein involved in the majority of spots detected by the staining method is actually settled in the range. Since minimum spot size being easily handled by our method appears to be about 2mm in diameter, as much as 30ng of protein in such a spot can be determined by the method. The method was used to follow the variation of chick serum proteins during embryonic development." @default.
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- W2488202112 date "1985-01-01" @default.
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- W2488202112 title "A Simple Method for Quantitative Analysis of Protein in Spots Separated by Two-Dimensional Polyacrylamide Gel Electrophoresis and Its Applications" @default.
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- W2488202112 doi "https://doi.org/10.1016/b978-0-08-031739-7.50236-6" @default.
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