FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2488294654> ?p ?o ?g. }

• W2488294654 endingPage "112" @default.
• W2488294654 startingPage "75" @default.
• W2488294654 abstract "Monoclonal antibodies are an important class of therapeutic agent that are in widespread use for the effective treatment of many human diseases. We have used a number of mass spectrometers (Synapt G2 and OrbiTrap Exactive Plus extended mass range [EMR]) and ion mobility instruments (Synapt G1 modified to an radio frequency [RF]-confining drift cell and a Synapt G2 travelling wave system) to characterise the humanised IgG1k NIST monoclonal antibody molecule under native-MS and buffer conditions. On both the Synapt G2 and the OrbiTrap instrumentation, charge state distributions are very similar, and all major glycoforms were resolved on all observable charge states; however, only on the OrbiTrap instrument are the glycoforms fully resolved to baseline. Ion mobility measurements were made in both helium and nitrogen drift gases on an RF-confining drift cell device with derived collision cross-section values for charge states +21 to +26 range from 6696 Å2 to 6892 Å2 in helium and 7223 Å2 to 7403 Å2 in nitrogen, respectively. This small but gradual increase in collision cross section with increasing charge can be attributed to a combination of increasing ion-induced dipole interaction between the charged protein and the neutral drift gas and the enlargement of the protein due to the repulsion of the surface charges. The higher charge states (+27 to +29) show a significant amount of gas-phase unfolding, evident by increased collision cross-section values and bimodal ion mobility arrival times. Collision cross sections measured on a travelling wave device (in nitrogen) were consistent with the mobility measurements made in nitrogen on the RF-confining drift cell device. Molecular dynamic simulations on the theoretically generated NIST monoclonal antibody (NISTmAb) coordinate structure indicate that the gas-phase structure undergoes a significant (up to 40%) amount of compaction. Theoretical collision cross-section calculations on the optimised molecular dynamic-derived NISTmAb structure are consistent with both helium and nitrogen instrument-derived collision cross-section values. Finally, collision-induced unfolding experiments also were performed on the +26 charge of the NISTmAb and could potentially be used as a means of providing structural information in addition to a single, and potentially limiting, gas phase-derived collision cross-section measurement." @default.
• W2488294654 created "2016-08-23" @default.
• W2488294654 creator A5008672082 @default.
• W2488294654 creator A5014110399 @default.
• W2488294654 creator A5019151198 @default.
• W2488294654 creator A5063275110 @default.
• W2488294654 date "2015-01-01" @default.
• W2488294654 modified "2023-10-17" @default.
• W2488294654 title "Ion Mobility and Mass Spectrometry Measurements of the Humanized IgGk NIST Monoclonal Antibody" @default.
• W2488294654 cites W1966037825 @default.
• W2488294654 cites W1966985858 @default.
• W2488294654 cites W1969724828 @default.
• W2488294654 cites W1970349972 @default.
• W2488294654 cites W1970471856 @default.
• W2488294654 cites W1973697545 @default.
• W2488294654 cites W1976727092 @default.
• W2488294654 cites W1978916713 @default.
• W2488294654 cites W1982468602 @default.
• W2488294654 cites W1983583299 @default.
• W2488294654 cites W1985004479 @default.
• W2488294654 cites W1988079335 @default.
• W2488294654 cites W1989567735 @default.
• W2488294654 cites W1991437941 @default.
• W2488294654 cites W1992935534 @default.
• W2488294654 cites W1994494141 @default.
• W2488294654 cites W1994628856 @default.
• W2488294654 cites W1997463565 @default.
• W2488294654 cites W1998116282 @default.
• W2488294654 cites W2005368152 @default.
• W2488294654 cites W2006910241 @default.
• W2488294654 cites W2011432577 @default.
• W2488294654 cites W2011859588 @default.
• W2488294654 cites W2013847834 @default.
• W2488294654 cites W2015855414 @default.
• W2488294654 cites W2016460201 @default.
• W2488294654 cites W2018808382 @default.
• W2488294654 cites W2019812098 @default.
• W2488294654 cites W2020266605 @default.
• W2488294654 cites W2022811603 @default.
• W2488294654 cites W2026183640 @default.
• W2488294654 cites W2027138806 @default.
• W2488294654 cites W2030175020 @default.
• W2488294654 cites W2033599685 @default.
• W2488294654 cites W2035123840 @default.
• W2488294654 cites W2035314417 @default.
• W2488294654 cites W2039125944 @default.
• W2488294654 cites W2043571416 @default.
• W2488294654 cites W2045138572 @default.
• W2488294654 cites W2049239108 @default.
• W2488294654 cites W2053866466 @default.
• W2488294654 cites W2054083856 @default.
• W2488294654 cites W2057009243 @default.
• W2488294654 cites W2057020790 @default.
• W2488294654 cites W2059903043 @default.
• W2488294654 cites W2061054232 @default.
• W2488294654 cites W2065544983 @default.
• W2488294654 cites W2071884490 @default.
• W2488294654 cites W2074901150 @default.
• W2488294654 cites W2078420278 @default.
• W2488294654 cites W2081249487 @default.
• W2488294654 cites W2084256518 @default.
• W2488294654 cites W2086145691 @default.
• W2488294654 cites W2087009324 @default.
• W2488294654 cites W2087242534 @default.
• W2488294654 cites W2090615391 @default.
• W2488294654 cites W2093645477 @default.
• W2488294654 cites W2096488752 @default.
• W2488294654 cites W2096587470 @default.
• W2488294654 cites W2097958897 @default.
• W2488294654 cites W2101821801 @default.
• W2488294654 cites W2102574135 @default.
• W2488294654 cites W2108791168 @default.
• W2488294654 cites W2112673374 @default.
• W2488294654 cites W2115569106 @default.
• W2488294654 cites W2115588498 @default.
• W2488294654 cites W2115763460 @default.
• W2488294654 cites W2123911097 @default.
• W2488294654 cites W2124816512 @default.
• W2488294654 cites W2125455336 @default.
• W2488294654 cites W2127267814 @default.
• W2488294654 cites W2129733824 @default.
• W2488294654 cites W2138014620 @default.
• W2488294654 cites W2142335003 @default.
• W2488294654 cites W2147443546 @default.
• W2488294654 cites W2156577106 @default.
• W2488294654 cites W2161300026 @default.
• W2488294654 cites W2165192566 @default.
• W2488294654 cites W2170167924 @default.
• W2488294654 cites W2170846952 @default.
• W2488294654 cites W2312841378 @default.
• W2488294654 cites W2314545667 @default.
• W2488294654 cites W2316249707 @default.
• W2488294654 cites W2317233863 @default.
• W2488294654 cites W2325522531 @default.
• W2488294654 cites W2326601291 @default.
• W2488294654 cites W2335645964 @default.
• W2488294654 cites W4244582831 @default.
• W2488294654 cites W4245569215 @default.

• next