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- W2488443674 abstract "Objective To analyze structural features of microtubules (MT) in ECV304 cell line after infection of dengue virus serotype 2 (DV-2) and investigate the role of MT in DV life cycles in the cells. Methods After ECV304 cells were infected with DV-2, viral antigen and MT were detected by double immunostaining. Colcemid, which disrupts MT, was added while DV began to release. The viral titer was measured by plaque assay. Results That MT randomly oriented in the cytoplasm and lost normal structure, MT ring around nucleus and MT bundle was observed during infection. Localization of DV-2 antigens was seen in the vicinity of MT aster. In the group treated with colcemid, the levels of extracellular and intracellular virus were higher than those in the group with infection alone. Conclusion DV-2 infection induced disruption of MT cytoskeleton and the disruption could enhance viral spreading." @default.
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- W2488443674 date "2005-01-01" @default.
- W2488443674 modified "2023-09-22" @default.
- W2488443674 title "Role of microtubules in dengue virus infection of ECV304 cell line" @default.
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