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- W2488968949 abstract "The cDNA of the green fluorescent protein gene (gfp) was amplified by PCR and expressed in Escherichia coli BL21 (DE23) by using pET-11C vector. A promoter-probe cassette with a strong SD sequence and the terminator of φ 10 gene from bacteriophage T7 was obtained. A gfp promoter probe vector, named pHN220, was constructed by the insertion of a BglII fragment containing the cassette into the unique BamHI site of pTR102. The unique BamHI site of the cassette was then used as the cloning site for Sau3AI generated DNA fragments.KeywordsEscherichia ColiPlant PhysiologyPromoter Activity21st CenturyBiological Nitrogen FixationThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
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- W2488968949 date "1998-01-01" @default.
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- W2488968949 title "Construction of a gfp Promoter-Probe Vector and its Application in the Study of Rhizobium fredii" @default.
- W2488968949 doi "https://doi.org/10.1007/978-94-011-5159-7_158" @default.
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