Matches in SemOpenAlex for { <https://semopenalex.org/work/W248898590> ?p ?o ?g. }
Showing items 1 to 41 of
41
with 100 items per page.
- W248898590 endingPage "393" @default.
- W248898590 startingPage "391" @default.
- W248898590 abstract "目的将乙型脑炎病毒NS1蛋白基因克隆至pET28-a（＋）表达载体,构建原核表达载体pET-NS1,并使该基因在E.coli中高效表达,为进一步研制乙脑早期诊断试剂奠定基础。方法根据GenBank中提供的乙型脑炎病毒SA14-14-2株全基因序列设计引物,通过反转录及巢式PCR方法扩增目的片段,测序后连接pET28-a（＋）载体,构建重组表达载体pET-NS1,转化大肠杆菌BL21（DE3）后,利用IPTG诱导获得高效表达。结果扩增出了1300bp的基因片段,与预期大小一致,测序后经Blast验证与已发表乙型脑炎病毒SA14-14-2株NS1蛋白基因序列同源性为100%,成功克隆至表达载体pET28-a（＋）并获得高效表达,表达产物分子量约为45kD,Western blot分析表明该表达产物具有良好抗原性。结论该表达产物的稳定高效表达及其抗原特异性为乙脑的诊断试剂开发提供了依据。" @default.
- W248898590 created "2016-06-24" @default.
- W248898590 creator A5025562220 @default.
- W248898590 creator A5032540186 @default.
- W248898590 creator A5036279331 @default.
- W248898590 creator A5046074893 @default.
- W248898590 creator A5054867771 @default.
- W248898590 creator A5057833769 @default.
- W248898590 creator A5061328704 @default.
- W248898590 creator A5065955866 @default.
- W248898590 creator A5086530456 @default.
- W248898590 date "2008-01-01" @default.
- W248898590 modified "2023-09-24" @default.
- W248898590 title "乙型脑炎病毒SA14-14-2株NS1蛋白基因在原核细胞中的高效表达及其表达产物的抗原性分析" @default.
- W248898590 hasPublicationYear "2008" @default.
- W248898590 type Work @default.
- W248898590 sameAs 248898590 @default.
- W248898590 citedByCount "0" @default.
- W248898590 crossrefType "journal-article" @default.
- W248898590 hasAuthorship W248898590A5025562220 @default.
- W248898590 hasAuthorship W248898590A5032540186 @default.
- W248898590 hasAuthorship W248898590A5036279331 @default.
- W248898590 hasAuthorship W248898590A5046074893 @default.
- W248898590 hasAuthorship W248898590A5054867771 @default.
- W248898590 hasAuthorship W248898590A5057833769 @default.
- W248898590 hasAuthorship W248898590A5061328704 @default.
- W248898590 hasAuthorship W248898590A5065955866 @default.
- W248898590 hasAuthorship W248898590A5086530456 @default.
- W248898590 hasConcept C144133560 @default.
- W248898590 hasConceptScore W248898590C144133560 @default.
- W248898590 hasIssue "4" @default.
- W248898590 hasLocation W2488985901 @default.
- W248898590 hasOpenAccess W248898590 @default.
- W248898590 hasPrimaryLocation W2488985901 @default.
- W248898590 hasVolume "33" @default.
- W248898590 isParatext "false" @default.
- W248898590 isRetracted "false" @default.
- W248898590 magId "248898590" @default.
- W248898590 workType "article" @default.