Matches in SemOpenAlex for { <https://semopenalex.org/work/W2489354069> ?p ?o ?g. }
- W2489354069 abstract "This chapter discusses the methods for histological localization of alcohol dehydrogenase. Tissue localization of multiple enzyme forms of human Alcohol Dehydrogenase (ADH) is of paramount importance to understand their physiological involvement in ethanol metabolism and organ susceptibility to alcohol damage. Histological techniques—such as immunohistochemistry and in situ hybridization, provide powerful and complementary approaches to identify definite cell layers or cell types that may contribute to ethanol elimination and, for that matter, be subjected to the local toxic effects of ethanol. Immunohistochemical methods rely on the availability of specific antibodies against different ADH forms. When polyclonal antiserum is the starting material, antibodies specific for a given ADH should be affinity purified and thoroughly checked for cross-reactivity against other ADH forms and proteins by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. In situ hybridization methods, being highly sensitive, should use specific homologous probes, which have been previously characterized by Northern blot analysis. With either technique, it is essential to perform adequate control experiments by using pre-immune serum, pre-absorbed antiserum or sense RNA probes. Experimental conditions should always be carefully optimized and it is advisable that results be validated with appropriate functional tests or activity assays." @default.
- W2489354069 created "2016-08-23" @default.
- W2489354069 creator A5001345932 @default.
- W2489354069 creator A5014995945 @default.
- W2489354069 creator A5021974002 @default.
- W2489354069 creator A5061929250 @default.
- W2489354069 creator A5073810500 @default.
- W2489354069 date "2005-01-01" @default.
- W2489354069 modified "2023-10-18" @default.
- W2489354069 title "Histological Localization of Alcohol Dehydrogenase: Methods, Approaches and Applications" @default.
- W2489354069 cites W1495595158 @default.
- W2489354069 cites W1497645148 @default.
- W2489354069 cites W1500082482 @default.
- W2489354069 cites W1516582884 @default.
- W2489354069 cites W1516693210 @default.
- W2489354069 cites W1595936654 @default.
- W2489354069 cites W1601172052 @default.
- W2489354069 cites W1603954828 @default.
- W2489354069 cites W1630520190 @default.
- W2489354069 cites W1641536023 @default.
- W2489354069 cites W1776021342 @default.
- W2489354069 cites W1891617153 @default.
- W2489354069 cites W1923205770 @default.
- W2489354069 cites W1964111101 @default.
- W2489354069 cites W1964460830 @default.
- W2489354069 cites W1968229022 @default.
- W2489354069 cites W1970168533 @default.
- W2489354069 cites W1971763515 @default.
- W2489354069 cites W1972293382 @default.
- W2489354069 cites W1972619806 @default.
- W2489354069 cites W1974209045 @default.
- W2489354069 cites W1974296240 @default.
- W2489354069 cites W1974680205 @default.
- W2489354069 cites W1976207992 @default.
- W2489354069 cites W1977465101 @default.
- W2489354069 cites W1978691650 @default.
- W2489354069 cites W1980143759 @default.
- W2489354069 cites W1980467354 @default.
- W2489354069 cites W1982281493 @default.
- W2489354069 cites W1983962635 @default.
- W2489354069 cites W1988059733 @default.
- W2489354069 cites W1989068033 @default.
- W2489354069 cites W1995369256 @default.
- W2489354069 cites W1998978968 @default.
- W2489354069 cites W1999937801 @default.
- W2489354069 cites W2000353675 @default.
- W2489354069 cites W2005614668 @default.
- W2489354069 cites W2005931649 @default.
- W2489354069 cites W2006206244 @default.
- W2489354069 cites W2010812610 @default.
- W2489354069 cites W2011966007 @default.
- W2489354069 cites W2012455477 @default.
- W2489354069 cites W2016041339 @default.
- W2489354069 cites W2016542848 @default.
- W2489354069 cites W2016976268 @default.
- W2489354069 cites W2021723651 @default.
- W2489354069 cites W2022663760 @default.
- W2489354069 cites W2022693991 @default.
- W2489354069 cites W2024269801 @default.
- W2489354069 cites W2024398506 @default.
- W2489354069 cites W2024964197 @default.
- W2489354069 cites W2031037761 @default.
- W2489354069 cites W2039426864 @default.
- W2489354069 cites W2039652688 @default.
- W2489354069 cites W2040120963 @default.
- W2489354069 cites W2041916914 @default.
- W2489354069 cites W2043421622 @default.
- W2489354069 cites W2043700110 @default.
- W2489354069 cites W2043747644 @default.
- W2489354069 cites W2046291076 @default.
- W2489354069 cites W2049087666 @default.
- W2489354069 cites W2049443931 @default.
- W2489354069 cites W2052892489 @default.
- W2489354069 cites W2053065522 @default.
- W2489354069 cites W2056371322 @default.
- W2489354069 cites W2057049383 @default.
- W2489354069 cites W2062479875 @default.
- W2489354069 cites W2062481410 @default.
- W2489354069 cites W2062741111 @default.
- W2489354069 cites W2062771570 @default.
- W2489354069 cites W2063210039 @default.
- W2489354069 cites W2064165498 @default.
- W2489354069 cites W2065847371 @default.
- W2489354069 cites W2067688826 @default.
- W2489354069 cites W2074050642 @default.
- W2489354069 cites W2074468139 @default.
- W2489354069 cites W2080134105 @default.
- W2489354069 cites W2082638707 @default.
- W2489354069 cites W2083091390 @default.
- W2489354069 cites W2083855388 @default.
- W2489354069 cites W2083955704 @default.
- W2489354069 cites W2084408569 @default.
- W2489354069 cites W2086157214 @default.
- W2489354069 cites W2087772480 @default.
- W2489354069 cites W2092593605 @default.
- W2489354069 cites W2092687502 @default.
- W2489354069 cites W2092909951 @default.
- W2489354069 cites W2095518791 @default.
- W2489354069 cites W2102787840 @default.
- W2489354069 cites W2103964659 @default.