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- W2489771663 abstract "Abstract Recent advances in site-specific gene editing technologies, split genetic systems, recombinases and transposon remobilization have been applied to disease vector mosquitoes with great success. These in turn now enable a suite of hypothesis-driven questions concerning the physiology and behaviour of important mosquito species, guided by previous advances in genome sequencing and transcriptomics. In this chapter, we will review progress in CRISPR/Cas9-based gene disruption, including target selection, mutation identification, tracking homology-dependent gene insertion, CRISPR-mediated gene drive mechanisms and CRISPR/Cas9 variants. We will also review progress in using split genetic systems such as Gal4/UAS, recombinase-mediated cassette exchange using ΦC31, as well as piggyBac-mediated forward genetic screens using transgene remobilization." @default.
- W2489771663 created "2016-08-23" @default.
- W2489771663 creator A5068857716 @default.
- W2489771663 creator A5088503493 @default.
- W2489771663 date "2016-01-01" @default.
- W2489771663 modified "2023-09-27" @default.
- W2489771663 title "Progress in Gene Editing Transgenesis Genome Manipulation in Mosquitoes" @default.
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