Matches in SemOpenAlex for { <https://semopenalex.org/work/W2490453670> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2490453670 endingPage "618a" @default.
- W2490453670 startingPage "617a" @default.
- W2490453670 abstract "Filopodia are finger-like protrusions at the leading edge of migrating cells and are crucial for cell motilities as antennae. It is known that actin filaments are bundled hexagonally and provide rigidity to filopodia by fascin, which is an actin filament bundling protein and plays a central role of actin bundling. However, molecular mechanisms of their formation have been still unclear. We, here, observed the filopodia of intact whole cells fixed by rapid freezing and revealed their three-dimensional maps by cryo-electron tomography and image processing; the actin filaments’ bundling structure was clarified at high resolutions under close-to-life conditions. It is found that the actin filaments in vivo were more numerous than those in the bundles reconstructed in vitro, whereas actin filaments and fascin possess dynamic properties in filopodia. In additions, each actin filament in the filopodia showed a slightly loose twist similar to those bundled by fascin in vitro. The cross-linking structures between actin filaments were also observed clearly and were determined to be fascin by comparison with actin and fascin atomic models. Then we identified three actin binding sites of fascin against two adjacent actin filaments: one bound fascin in two binding regions with wide surfaces and the other did in a tiny region. We propose that these two different binding manners between actin and fascin should provide rigid bundles but flexible and dynamic performance." @default.
- W2490453670 created "2016-08-23" @default.
- W2490453670 creator A5035463618 @default.
- W2490453670 creator A5065889159 @default.
- W2490453670 creator A5069327314 @default.
- W2490453670 creator A5085835967 @default.
- W2490453670 date "2016-02-01" @default.
- W2490453670 modified "2023-10-16" @default.
- W2490453670 title "Actin Filaments Bundling Mechanisms by Fascin in Filopodia were Revealed with Cryo-ET" @default.
- W2490453670 doi "https://doi.org/10.1016/j.bpj.2015.11.3314" @default.
- W2490453670 hasPublicationYear "2016" @default.
- W2490453670 type Work @default.
- W2490453670 sameAs 2490453670 @default.
- W2490453670 citedByCount "0" @default.
- W2490453670 crossrefType "journal-article" @default.
- W2490453670 hasAuthorship W2490453670A5035463618 @default.
- W2490453670 hasAuthorship W2490453670A5065889159 @default.
- W2490453670 hasAuthorship W2490453670A5069327314 @default.
- W2490453670 hasAuthorship W2490453670A5085835967 @default.
- W2490453670 hasBestOaLocation W24904536701 @default.
- W2490453670 hasConcept C12554922 @default.
- W2490453670 hasConcept C125705527 @default.
- W2490453670 hasConcept C14228908 @default.
- W2490453670 hasConcept C142669718 @default.
- W2490453670 hasConcept C1491633281 @default.
- W2490453670 hasConcept C185592680 @default.
- W2490453670 hasConcept C189040839 @default.
- W2490453670 hasConcept C199528912 @default.
- W2490453670 hasConcept C2778934503 @default.
- W2490453670 hasConcept C2993400109 @default.
- W2490453670 hasConcept C3620293 @default.
- W2490453670 hasConcept C55493867 @default.
- W2490453670 hasConcept C59999672 @default.
- W2490453670 hasConcept C86803240 @default.
- W2490453670 hasConcept C90301020 @default.
- W2490453670 hasConcept C95444343 @default.
- W2490453670 hasConceptScore W2490453670C12554922 @default.
- W2490453670 hasConceptScore W2490453670C125705527 @default.
- W2490453670 hasConceptScore W2490453670C14228908 @default.
- W2490453670 hasConceptScore W2490453670C142669718 @default.
- W2490453670 hasConceptScore W2490453670C1491633281 @default.
- W2490453670 hasConceptScore W2490453670C185592680 @default.
- W2490453670 hasConceptScore W2490453670C189040839 @default.
- W2490453670 hasConceptScore W2490453670C199528912 @default.
- W2490453670 hasConceptScore W2490453670C2778934503 @default.
- W2490453670 hasConceptScore W2490453670C2993400109 @default.
- W2490453670 hasConceptScore W2490453670C3620293 @default.
- W2490453670 hasConceptScore W2490453670C55493867 @default.
- W2490453670 hasConceptScore W2490453670C59999672 @default.
- W2490453670 hasConceptScore W2490453670C86803240 @default.
- W2490453670 hasConceptScore W2490453670C90301020 @default.
- W2490453670 hasConceptScore W2490453670C95444343 @default.
- W2490453670 hasIssue "3" @default.
- W2490453670 hasLocation W24904536701 @default.
- W2490453670 hasOpenAccess W2490453670 @default.
- W2490453670 hasPrimaryLocation W24904536701 @default.
- W2490453670 hasRelatedWork W1989895836 @default.
- W2490453670 hasRelatedWork W1997998898 @default.
- W2490453670 hasRelatedWork W2035918801 @default.
- W2490453670 hasRelatedWork W2103431046 @default.
- W2490453670 hasRelatedWork W2122845371 @default.
- W2490453670 hasRelatedWork W2143561574 @default.
- W2490453670 hasRelatedWork W2376601679 @default.
- W2490453670 hasRelatedWork W2490453670 @default.
- W2490453670 hasRelatedWork W2913003816 @default.
- W2490453670 hasRelatedWork W4319915897 @default.
- W2490453670 hasVolume "110" @default.
- W2490453670 isParatext "false" @default.
- W2490453670 isRetracted "false" @default.
- W2490453670 magId "2490453670" @default.
- W2490453670 workType "article" @default.