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- W2491472563 abstract "A high rate of plastome-encoded mutations was induced in Nicotiana by exposing seeds to N-nitroso-N- methylurea. Seeds then were subjected to nutritional and in vitro selection procedures for systematic isolation of plastome- dependent antibiotic-resistant plants. Multiple flowering lines resistant to streptomycin, spectinomycin, lincomycin, or chloramphenicol were obtained. A detailed analysis of the streptomycin-resistant lines is presented. Sexual hybridiza- tion, cybrid formation following protoplast fusion, and in or- ganello protein synthesis were used to rigorously assign the mutations to the chloroplast genome. The efficient rates of mutagenesis combined with the in vitro mass-screening proce- dures described here should facilitate investigation of funda- mental aspects of chloroplast genetics in higher plants." @default.
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- W2491472563 date "2016-01-01" @default.
- W2491472563 modified "2023-09-26" @default.
- W2491472563 title "Efficient induction and selection of resistant mutants in Nicotiana (N-nitroso-N-methylurea/plant cell culture/protoplast fusion/in organ" @default.
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