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- W2491661515 abstract "Publisher Summary This chapter presents an examination of the mechanism of inhibition and activation of phosphofructokinase (PFK). On examining the ATP inhibition of Novikoff ascites tumor PFK, a lag in the onset of inhibition by ATP was observed. PFK was assayed in the presence of 3 mM ATP and 0.06 mM F-6-P. The concentrations of P i or AMP required for maximal stimulation of PFK were examined to determine whether P i and AMP act at the same or different sites on PFK. It was of interest to determine whether ATP or the ATP–Mg 2+ complex was the true inhibitor of PFK. No irreversible inactivation of PFK occurred during the preincubation and the assay period, because the subsequent addition of P i and AMP restored the enzyme activity to the level found prior to the preincubation. Experiments under way are designed to examine other inhibitors and activators in relation to the proposed schemes." @default.
- W2491661515 created "2016-08-23" @default.
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- W2491661515 date "1965-01-01" @default.
- W2491661515 modified "2023-10-12" @default.
- W2491661515 title "ON THE MECHANISMS OF INHIBITION AND ACTIVATION OF PHOSPHOFRUCTOKINASE IN NOVIKOFF ASCITES TUMOR CELLS**This investigation was supported by Public Heatlh Service Research Grant No. CA-05706 from the National Cancer Institute." @default.
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- W2491661515 doi "https://doi.org/10.1016/b978-1-4832-3161-7.50016-1" @default.
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