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• W2492844898 abstract "Salmonella enterica serovar Enteritidis is one of the most frequently reported causes of foodborne illness. A rapid and sensitive detection of the insertion element (Iel) gene of Salmonella Enteritidis on screen-printed carbon electrodes (SPCE) is reported in this paper. The biosensor system includes two nanoparticles: gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs). The AuNPs are coated with the 1st target-specific DNA probe (pDNA) which can recognize the target DNA (tDNA) and the MNPs are coated with the 2nd target-specific pDNA. After mixing the nanoparticles with the tDNA, the sandwich complex (MNP-2ndpDNA/tDNA/1stpDNA-AuNP) is formed. The whole complex is magnetically separated and applied on a screen-printed carbon electrode chip after washing away the unreacted AuNPs. Following oxidative gold metal dissolution in an acidic solution, the released Au3+ ions are directly quantified by differential pulse voltammetry (DPV) at a potential of +0.4 V (vs. Ag/AgCl). Using this technique, the detection limit of this SPCE biosensor for the insertion element (Iel) gene of Salmonella Enteritidis is as low as 0.7 ng/mL." @default.
• W2492844898 created "2016-08-23" @default.
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• W2492844898 date "2013-01-01" @default.
• W2492844898 modified "2023-09-26" @default.
• W2492844898 title "AuNP-DNA Biosensor for Rapid Detection of Salmonella enterica Serovar Enteritidis" @default.
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• W2492844898 doi "https://doi.org/10.1021/bk-2013-1143.ch003" @default.
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