Matches in SemOpenAlex for { <https://semopenalex.org/work/W2494213882> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2494213882 abstract "To explore the variability in biosensor studies, 150participants from 20 countries were given the same proteinsamples and asked to determine kinetic rate constants for theinteraction. We chose a protein system that was amenable toanalysis using different biosensor platforms as well as byusers of different expertise levels. The two proteins (a 50-kDaFab and a 60-kDa glutathione S-transferase [GST] antigen) forma relatively high-affinity complex, so participants needed tooptimize several experimental parameters, including ligandimmobilization and regeneration conditions as well as analyteconcentrations and injection/dissociation times. Although mostparticipants collected binding responses that could be fit toyield kinetic parameters, the quality of a few data sets couldhave been improved by optimizing the assay design. Once theseoutliers were removed, the average reported affinity across theremaining panel of participants was 620 pM with a standarddeviation of 980 pM. These results demonstrate that when thisbiosensor assay was designed and executed appropriately, thereported rate constants were consistent, and independent ofwhich protein was immobilized and which biosensor was used." @default.
- W2494213882 created "2016-08-23" @default.
- W2494213882 creator A5011789826 @default.
- W2494213882 creator A5050770494 @default.
- W2494213882 creator A5066137013 @default.
- W2494213882 creator A5078912603 @default.
- W2494213882 date "2009-01-01" @default.
- W2494213882 modified "2023-09-24" @default.
- W2494213882 title "A global benchmark study using affinity-based biosensors" @default.
- W2494213882 hasPublicationYear "2009" @default.
- W2494213882 type Work @default.
- W2494213882 sameAs 2494213882 @default.
- W2494213882 citedByCount "0" @default.
- W2494213882 crossrefType "journal-article" @default.
- W2494213882 hasAuthorship W2494213882A5011789826 @default.
- W2494213882 hasAuthorship W2494213882A5050770494 @default.
- W2494213882 hasAuthorship W2494213882A5066137013 @default.
- W2494213882 hasAuthorship W2494213882A5078912603 @default.
- W2494213882 hasConcept C10390740 @default.
- W2494213882 hasConcept C105795698 @default.
- W2494213882 hasConcept C113196181 @default.
- W2494213882 hasConcept C160756335 @default.
- W2494213882 hasConcept C170493617 @default.
- W2494213882 hasConcept C179247698 @default.
- W2494213882 hasConcept C181199279 @default.
- W2494213882 hasConcept C185592680 @default.
- W2494213882 hasConcept C21951064 @default.
- W2494213882 hasConcept C33923547 @default.
- W2494213882 hasConcept C43617362 @default.
- W2494213882 hasConcept C55493867 @default.
- W2494213882 hasConcept C74998103 @default.
- W2494213882 hasConcept C79337645 @default.
- W2494213882 hasConceptScore W2494213882C10390740 @default.
- W2494213882 hasConceptScore W2494213882C105795698 @default.
- W2494213882 hasConceptScore W2494213882C113196181 @default.
- W2494213882 hasConceptScore W2494213882C160756335 @default.
- W2494213882 hasConceptScore W2494213882C170493617 @default.
- W2494213882 hasConceptScore W2494213882C179247698 @default.
- W2494213882 hasConceptScore W2494213882C181199279 @default.
- W2494213882 hasConceptScore W2494213882C185592680 @default.
- W2494213882 hasConceptScore W2494213882C21951064 @default.
- W2494213882 hasConceptScore W2494213882C33923547 @default.
- W2494213882 hasConceptScore W2494213882C43617362 @default.
- W2494213882 hasConceptScore W2494213882C55493867 @default.
- W2494213882 hasConceptScore W2494213882C74998103 @default.
- W2494213882 hasConceptScore W2494213882C79337645 @default.
- W2494213882 hasIssue "2" @default.
- W2494213882 hasLocation W24942138821 @default.
- W2494213882 hasOpenAccess W2494213882 @default.
- W2494213882 hasPrimaryLocation W24942138821 @default.
- W2494213882 hasRelatedWork W1949102410 @default.
- W2494213882 hasRelatedWork W2006695977 @default.
- W2494213882 hasRelatedWork W2066784367 @default.
- W2494213882 hasRelatedWork W2098380717 @default.
- W2494213882 hasRelatedWork W2160984334 @default.
- W2494213882 hasRelatedWork W240699860 @default.
- W2494213882 hasRelatedWork W2408009935 @default.
- W2494213882 hasRelatedWork W2461849488 @default.
- W2494213882 hasRelatedWork W2972694687 @default.
- W2494213882 isParatext "false" @default.
- W2494213882 isRetracted "false" @default.
- W2494213882 magId "2494213882" @default.
- W2494213882 workType "article" @default.