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- W2494822475 endingPage "pp.00950.2016" @default.
- W2494822475 startingPage "pp.00950.2016" @default.
- W2494822475 abstract "The storage compounds are deposited into plant seeds during maturation. As the model oilseed species, Arabidopsis (Arabidopsis thaliana) has long been studied for seed oil deposition. However, the regulation of this process remains unclear. Through genetic screen with a seed oil body-specific reporter, we isolated low oil1 (loo1) mutant. LOO1 was mapped to HISTIDINE BIOSYNTHESIS NUMBER 1A (HISN1A). HISN1A catalyzes the first step of His biosynthesis. Oil significantly decreased, and conversely proteins markedly increased in hisn1a mutants, indicating that HISN1A regulates both oil accumulation and the oil-protein balance. HISN1A was predominantly expressed in embryos and root tips. Accordingly, the hisn1a mutants exhibited developmental phenotype especially of seeds and roots. Transcriptional profiling displayed that β-oxidation was the major metabolic pathway downstream of HISN1A. β-Oxidation was induced in hisn1a mutants, whereas it was reduced in 35S:HISN1A-transgenic plants. In plants, seed storage oil is broken-down by β-oxidation, which is controlled by abscisic acid (ABA). We found that His activated genes of ABA biosynthesis and correspondingly advanced ABA accumulation. Exogenous ABA rescued the defects of hisn1a mutants, whereas mutation of ABA DEFICIENT2, a key enzyme in ABA biosynthesis, blocked the effect of His on β-oxidation, indicating that ABA mediates His regulation in β-oxidation. Intriguingly, structural analysis showed that a potential His-binding domain was present in the general amino acid sensors GENERAL CONTROL NON-DEREPRESSIBLE2 and PII, suggesting that His may serve as a signal molecule. Taken together, our study reveals that His promotes plant seed oil deposition through ABA biosynthesis and β-oxidation." @default.
- W2494822475 created "2016-08-23" @default.
- W2494822475 creator A5010137084 @default.
- W2494822475 creator A5031110963 @default.
- W2494822475 date "2016-08-04" @default.
- W2494822475 modified "2023-09-27" @default.
- W2494822475 title "Histidine Regulates Seed Oil Deposition through Abscisic Acid Biosynthesis and β-oxidation" @default.
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- W2494822475 doi "https://doi.org/10.1104/pp.16.00950" @default.
- W2494822475 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5047104" @default.
- W2494822475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27493214" @default.
- W2494822475 hasPublicationYear "2016" @default.
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