FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2494866919> ?p ?o ?g. }

• W2494866919 endingPage "1509" @default.
• W2494866919 startingPage "1500" @default.
• W2494866919 abstract "Yeast tRNA ligase (Trl1) is an essential trifunctional enzyme that repairs RNA breaks with 2′,3′-cyclic-PO 4 and 5′-OH ends. Trl1 is composed of C-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends to generate the 3′-OH, 2′-PO 4 , and 5′-PO 4 termini required for sealing by an N-terminal ligase domain. Trl1 enzymes are found in all human fungal pathogens and they are promising targets for antifungal drug discovery because: (i) their domain structures and biochemical mechanisms are unique compared to the mammalian RtcB-type tRNA splicing enzyme; and (ii) there are no obvious homologs of the Trl1 ligase domain in mammalian proteomes. Here we characterize the tRNA ligases of two human fungal pathogens: Coccidioides immitis and Aspergillus fumigatus . The biological activity of CimTrl1 and AfuTrl1 was verified by showing that their expression complements a Saccharomyces cerevisiae trl1 Δ mutant . Purified recombinant AfuTrl1 and CimTrl1 proteins were catalytically active in joining 2′,3′-cyclic-PO 4 and 5′-OH ends in vitro, either as full-length proteins or as a mixture of separately produced healing and sealing domains. The biochemical properties of CimTrl1 and AfuTrl1 are similar to those of budding yeast Trl1, particularly with respect to their preferential use of GTP as the phosphate donor for the polynucleotide kinase reaction. Our findings provide genetic and biochemical tools to screen for inhibitors of tRNA ligases from pathogenic fungi." @default.
• W2494866919 created "2016-08-23" @default.
• W2494866919 creator A5014112082 @default.
• W2494866919 creator A5026038727 @default.
• W2494866919 creator A5065368802 @default.
• W2494866919 date "2016-08-04" @default.
• W2494866919 modified "2023-10-14" @default.
• W2494866919 title "Characterization of the tRNA ligases of pathogenic fungi <i>Aspergillus fumigatus</i> and <i>Coccidioides immitis</i>" @default.
• W2494866919 cites W1497913243 @default.
• W2494866919 cites W1558517979 @default.
• W2494866919 cites W1603833582 @default.
• W2494866919 cites W1754380718 @default.
• W2494866919 cites W1803102843 @default.
• W2494866919 cites W1970997159 @default.
• W2494866919 cites W1974095433 @default.
• W2494866919 cites W1989843311 @default.
• W2494866919 cites W1997757991 @default.
• W2494866919 cites W1997816658 @default.
• W2494866919 cites W2019583554 @default.
• W2494866919 cites W2020066001 @default.
• W2494866919 cites W2033387234 @default.
• W2494866919 cites W2038538652 @default.
• W2494866919 cites W2052928602 @default.
• W2494866919 cites W2060084030 @default.
• W2494866919 cites W2065232441 @default.
• W2494866919 cites W2070420045 @default.
• W2494866919 cites W2082956579 @default.
• W2494866919 cites W2099685008 @default.
• W2494866919 cites W2104648145 @default.
• W2494866919 cites W2113984566 @default.
• W2494866919 cites W2117218702 @default.
• W2494866919 cites W2141197648 @default.
• W2494866919 cites W2142033846 @default.
• W2494866919 cites W2143576961 @default.
• W2494866919 cites W2154686047 @default.
• W2494866919 cites W2225337637 @default.
• W2494866919 cites W4251007507 @default.
• W2494866919 doi "https://doi.org/10.1261/rna.057455.116" @default.
• W2494866919 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5029449" @default.
• W2494866919 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27492257" @default.
• W2494866919 hasPublicationYear "2016" @default.
• W2494866919 type Work @default.
• W2494866919 sameAs 2494866919 @default.
• W2494866919 citedByCount "9" @default.
• W2494866919 countsByYear W24948669192017 @default.
• W2494866919 countsByYear W24948669192018 @default.
• W2494866919 countsByYear W24948669192019 @default.
• W2494866919 countsByYear W24948669192021 @default.
• W2494866919 crossrefType "journal-article" @default.
• W2494866919 hasAuthorship W2494866919A5014112082 @default.
• W2494866919 hasAuthorship W2494866919A5026038727 @default.
• W2494866919 hasAuthorship W2494866919A5065368802 @default.
• W2494866919 hasBestOaLocation W24948669191 @default.
• W2494866919 hasConcept C104317684 @default.
• W2494866919 hasConcept C134459356 @default.
• W2494866919 hasConcept C153957851 @default.
• W2494866919 hasConcept C175114707 @default.
• W2494866919 hasConcept C181199279 @default.
• W2494866919 hasConcept C25602115 @default.
• W2494866919 hasConcept C2777576037 @default.
• W2494866919 hasConcept C2778439347 @default.
• W2494866919 hasConcept C2779222958 @default.
• W2494866919 hasConcept C2779787849 @default.
• W2494866919 hasConcept C2781030883 @default.
• W2494866919 hasConcept C2910135453 @default.
• W2494866919 hasConcept C55493867 @default.
• W2494866919 hasConcept C67705224 @default.
• W2494866919 hasConcept C86803240 @default.
• W2494866919 hasConcept C89423630 @default.
• W2494866919 hasConceptScore W2494866919C104317684 @default.
• W2494866919 hasConceptScore W2494866919C134459356 @default.
• W2494866919 hasConceptScore W2494866919C153957851 @default.
• W2494866919 hasConceptScore W2494866919C175114707 @default.
• W2494866919 hasConceptScore W2494866919C181199279 @default.
• W2494866919 hasConceptScore W2494866919C25602115 @default.
• W2494866919 hasConceptScore W2494866919C2777576037 @default.
• W2494866919 hasConceptScore W2494866919C2778439347 @default.
• W2494866919 hasConceptScore W2494866919C2779222958 @default.
• W2494866919 hasConceptScore W2494866919C2779787849 @default.
• W2494866919 hasConceptScore W2494866919C2781030883 @default.
• W2494866919 hasConceptScore W2494866919C2910135453 @default.
• W2494866919 hasConceptScore W2494866919C55493867 @default.
• W2494866919 hasConceptScore W2494866919C67705224 @default.
• W2494866919 hasConceptScore W2494866919C86803240 @default.
• W2494866919 hasConceptScore W2494866919C89423630 @default.
• W2494866919 hasFunder F4320332161 @default.
• W2494866919 hasFunder F4320337351 @default.
• W2494866919 hasIssue "10" @default.
• W2494866919 hasLocation W24948669191 @default.
• W2494866919 hasLocation W24948669192 @default.
• W2494866919 hasLocation W24948669193 @default.
• W2494866919 hasLocation W24948669194 @default.
• W2494866919 hasOpenAccess W2494866919 @default.
• W2494866919 hasPrimaryLocation W24948669191 @default.
• W2494866919 hasRelatedWork W1999763554 @default.
• W2494866919 hasRelatedWork W2007305755 @default.
• W2494866919 hasRelatedWork W2023555243 @default.
• W2494866919 hasRelatedWork W2026244176 @default.

• next