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- W2495196935 abstract "We have developed a simple analytical method for the detection of mercuric ions at low ppb levels. We combined inexpensive ELISA methodology with the selective, high affinity recognition of Hg2+ ions by dithiocarbamate chelators. Thus, we employed sulfur-containing complexing agents instead of antibodies as analytical tools. One of our assay formats comprises a sandwich chelate formed by a chelator doped on the surface of the ELISA plate, the mercuric ion, and a chelator coupled to a reporter enzyme. Another format is based on the competition of the analyte ions with a mercury containing reagent (e.g., enzyme tracer) for the binding to a chelate linked reagent (e.g., immobilized chelator). Our preliminary results demonstrate that the high sensitivity and selectivity of our assay systems hold promise for the monitoring of mercury in the environment." @default.
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- W2495196935 date "1995-03-23" @default.
- W2495196935 modified "2023-09-25" @default.
- W2495196935 title "Development of Assay for Analysis of Hg<sup>2+</sup> Based on Sulfur-Containing Ligands" @default.
- W2495196935 doi "https://doi.org/10.1021/bk-1995-0586.ch018" @default.
- W2495196935 hasPublicationYear "1995" @default.
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