Matches in SemOpenAlex for { <https://semopenalex.org/work/W2495286952> ?p ?o ?g. }
- W2495286952 endingPage "2776" @default.
- W2495286952 startingPage "2767" @default.
- W2495286952 abstract "Endothelial cells (ECs) play a crucial role in regulating various physiological and pathological processes. The behavior of ECs is modulated by physical (e.g., substrate stiffness) and biochemical cues (e.g., growth factors). However, the synergistic influence of these cues on EC behavior has rarely been investigated. In this study, we constructed poly(l-lysine)/hyaluronan (PLL/HA) multilayer films with different stiffness and exposed ECs to these substrates with and without hepatocyte growth factor (HGF)-supplemented culture medium. We demonstrated that EC adhesion, migration, and proliferation were positively correlated with substrate stiffness and that these behaviors were further promoted by HGF. Interestingly, ECs on the lower stiffness substrates showed stronger responses to HGF in terms of migration and proliferation, suggesting that HGF can profoundly influence stiffness-dependent EC behavior correlated with EC growth. After the formation of an EC monolayer, EC behaviors correlated with endothelial function were evaluated by characterizing monolayer integrity, nitric oxide production, and gene expression of endothelial nitric oxide synthase. For the first time, we demonstrated that endothelial function displayed a negative correlation with substrate stiffness. Although HGF improved endothelial function, HGF was not able to change the stiffness-dependent manner of endothelial functions. Taken together, this study provides insights into the synergetic influence of physical and biochemical cues on EC behavior and offers great potential in the development of optimized biomaterials for EC-based regenerative medicine." @default.
- W2495286952 created "2016-08-23" @default.
- W2495286952 creator A5007028732 @default.
- W2495286952 creator A5008188330 @default.
- W2495286952 creator A5010931954 @default.
- W2495286952 creator A5015625537 @default.
- W2495286952 creator A5061219770 @default.
- W2495286952 creator A5064323494 @default.
- W2495286952 creator A5071891848 @default.
- W2495286952 creator A5078435985 @default.
- W2495286952 creator A5083023993 @default.
- W2495286952 creator A5086389109 @default.
- W2495286952 date "2016-08-02" @default.
- W2495286952 modified "2023-10-13" @default.
- W2495286952 title "Substrate Stiffness Combined with Hepatocyte Growth Factor Modulates Endothelial Cell Behavior" @default.
- W2495286952 cites W1497340615 @default.
- W2495286952 cites W1522985778 @default.
- W2495286952 cites W1574424832 @default.
- W2495286952 cites W1643339131 @default.
- W2495286952 cites W171904674 @default.
- W2495286952 cites W1789606850 @default.
- W2495286952 cites W1821824803 @default.
- W2495286952 cites W1912972578 @default.
- W2495286952 cites W1936770188 @default.
- W2495286952 cites W1962348298 @default.
- W2495286952 cites W1984846299 @default.
- W2495286952 cites W1985556486 @default.
- W2495286952 cites W1985562847 @default.
- W2495286952 cites W1987846826 @default.
- W2495286952 cites W1988871507 @default.
- W2495286952 cites W1990688945 @default.
- W2495286952 cites W1993006648 @default.
- W2495286952 cites W1998849298 @default.
- W2495286952 cites W1999666701 @default.
- W2495286952 cites W2004648940 @default.
- W2495286952 cites W2029167783 @default.
- W2495286952 cites W2032780005 @default.
- W2495286952 cites W2033638350 @default.
- W2495286952 cites W2033764325 @default.
- W2495286952 cites W2036034164 @default.
- W2495286952 cites W2037703016 @default.
- W2495286952 cites W2039110457 @default.
- W2495286952 cites W2042324535 @default.
- W2495286952 cites W2045856259 @default.
- W2495286952 cites W2047857252 @default.
- W2495286952 cites W2054635074 @default.
- W2495286952 cites W2063212208 @default.
- W2495286952 cites W2065412432 @default.
- W2495286952 cites W2066574979 @default.
- W2495286952 cites W2067776924 @default.
- W2495286952 cites W2069421644 @default.
- W2495286952 cites W2075356757 @default.
- W2495286952 cites W2078429451 @default.
- W2495286952 cites W2085760392 @default.
- W2495286952 cites W2088841619 @default.
- W2495286952 cites W2095545554 @default.
- W2495286952 cites W2097463592 @default.
- W2495286952 cites W2106258544 @default.
- W2495286952 cites W2107233004 @default.
- W2495286952 cites W2110926368 @default.
- W2495286952 cites W2111250483 @default.
- W2495286952 cites W2116159025 @default.
- W2495286952 cites W2116379215 @default.
- W2495286952 cites W2118260907 @default.
- W2495286952 cites W2121269537 @default.
- W2495286952 cites W2125174004 @default.
- W2495286952 cites W2128273788 @default.
- W2495286952 cites W2134208433 @default.
- W2495286952 cites W2152755217 @default.
- W2495286952 cites W2160250307 @default.
- W2495286952 cites W2164530385 @default.
- W2495286952 cites W2165673443 @default.
- W2495286952 cites W2167645403 @default.
- W2495286952 cites W2168857275 @default.
- W2495286952 cites W2315800446 @default.
- W2495286952 cites W2318473401 @default.
- W2495286952 cites W2324080600 @default.
- W2495286952 cites W2324482901 @default.
- W2495286952 cites W2599199563 @default.
- W2495286952 doi "https://doi.org/10.1021/acs.biomac.6b00318" @default.
- W2495286952 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5024748" @default.
- W2495286952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27428305" @default.
- W2495286952 hasPublicationYear "2016" @default.
- W2495286952 type Work @default.
- W2495286952 sameAs 2495286952 @default.
- W2495286952 citedByCount "35" @default.
- W2495286952 countsByYear W24952869522017 @default.
- W2495286952 countsByYear W24952869522018 @default.
- W2495286952 countsByYear W24952869522019 @default.
- W2495286952 countsByYear W24952869522020 @default.
- W2495286952 countsByYear W24952869522021 @default.
- W2495286952 countsByYear W24952869522022 @default.
- W2495286952 countsByYear W24952869522023 @default.
- W2495286952 crossrefType "journal-article" @default.
- W2495286952 hasAuthorship W2495286952A5007028732 @default.
- W2495286952 hasAuthorship W2495286952A5008188330 @default.
- W2495286952 hasAuthorship W2495286952A5010931954 @default.
- W2495286952 hasAuthorship W2495286952A5015625537 @default.