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- W2495802012 abstract "A miniaturized and self-contained in situ genetic analyzer Integrated In Situ Analyzer-Gene (IISA-Gene) was developed to conduct PCR-based targeted sequence detection using microfluidic technology. The core element of the in situ analyzer is a microfluidic device that conducts cell lysis, DNA purification, PCR, and optical detection of amplified DNA fragments. In this device, a standard three-step SYBR green PCR amplification with a pair of oligonucleotide primers is conducted using environmental microbial DNA templates eluted from glass beads packed in a microchannel. The microfluidic device is integrated with pumping components and control electronics for real-field deployments. Here, the performance of the microfluidic device and results of in situ operation of the IISA-Gene in deep-sea hydrothermal areas are presented." @default.
- W2495802012 created "2016-08-23" @default.
- W2495802012 creator A5078470183 @default.
- W2495802012 creator A5082149446 @default.
- W2495802012 date "2012-01-01" @default.
- W2495802012 modified "2023-10-11" @default.
- W2495802012 title "Microfabricated Flow-Through Device for In Situ Gene Analysis" @default.
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- W2495802012 doi "https://doi.org/10.1007/978-1-61779-915-0_3" @default.
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