Matches in SemOpenAlex for { <https://semopenalex.org/work/W2495993102> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2495993102 endingPage "86" @default.
- W2495993102 startingPage "67" @default.
- W2495993102 abstract "Industrial scale purification of therapeutically useful antibodies (immunoglobulin, IgGs) typically rely on Protein A affinity chromatography, as a first step. Eluents i.e aqueous solution of small organic molecules, are typically used to elute the antibodies from the affinity column. The molecular interactions between the antibody and the Protein A affinity resin are exceedingly dependent on pH and eluent concentration. This complex mechanism is not well understood, beyond the application of general trends, for eluents such as arginine and citrate. We have performed molecular mechanics simulations of antibody and Protein A to rationalize the experimental observations of the effects of arginine and citrate on purification yield. It was found that the highly positively charged arginine reduced the binding free energy (ΔΔGB) between the Protein A affinity resin and the antibody. While the negatively charged citrate increases the ΔΔGB for Protein A affinity resin and the antibody. The results provide a rationale for the observed fluctuations in the recovery of antibodies using citrate and arginine, and also provided insight into of the nature of the molecular interactions, which can be exploited for eluent design on Protein A affinity chromatography." @default.
- W2495993102 created "2016-08-23" @default.
- W2495993102 creator A5000143645 @default.
- W2495993102 creator A5053940473 @default.
- W2495993102 date "2013-01-01" @default.
- W2495993102 modified "2023-09-23" @default.
- W2495993102 title "Understanding the Role of Arginine and Citrate as Eluents in Affinity Chromatography" @default.
- W2495993102 cites W1976499671 @default.
- W2495993102 cites W1982762997 @default.
- W2495993102 cites W1984338378 @default.
- W2495993102 cites W1987920176 @default.
- W2495993102 cites W1997723318 @default.
- W2495993102 cites W2001439998 @default.
- W2495993102 cites W2009698511 @default.
- W2495993102 cites W2016775291 @default.
- W2495993102 cites W2017643816 @default.
- W2495993102 cites W2018033335 @default.
- W2495993102 cites W2022548497 @default.
- W2495993102 cites W2031672665 @default.
- W2495993102 cites W2032406779 @default.
- W2495993102 cites W2035651421 @default.
- W2495993102 cites W2045596349 @default.
- W2495993102 cites W2047647822 @default.
- W2495993102 cites W2053850848 @default.
- W2495993102 cites W2058008956 @default.
- W2495993102 cites W2067174909 @default.
- W2495993102 cites W2074838864 @default.
- W2495993102 cites W2096387523 @default.
- W2495993102 cites W2150981663 @default.
- W2495993102 cites W2162166182 @default.
- W2495993102 cites W4253774994 @default.
- W2495993102 cites W4293253343 @default.
- W2495993102 doi "https://doi.org/10.1021/bk-2013-1125.ch005" @default.
- W2495993102 hasPublicationYear "2013" @default.
- W2495993102 type Work @default.
- W2495993102 sameAs 2495993102 @default.
- W2495993102 citedByCount "1" @default.
- W2495993102 countsByYear W24959931022023 @default.
- W2495993102 crossrefType "book-chapter" @default.
- W2495993102 hasAuthorship W2495993102A5000143645 @default.
- W2495993102 hasAuthorship W2495993102A5053940473 @default.
- W2495993102 hasConcept C134121241 @default.
- W2495993102 hasConcept C159654299 @default.
- W2495993102 hasConcept C178790620 @default.
- W2495993102 hasConcept C179247698 @default.
- W2495993102 hasConcept C181199279 @default.
- W2495993102 hasConcept C184651966 @default.
- W2495993102 hasConcept C185592680 @default.
- W2495993102 hasConcept C191897082 @default.
- W2495993102 hasConcept C192562407 @default.
- W2495993102 hasConcept C203014093 @default.
- W2495993102 hasConcept C2777468819 @default.
- W2495993102 hasConcept C2779000548 @default.
- W2495993102 hasConcept C43617362 @default.
- W2495993102 hasConcept C515207424 @default.
- W2495993102 hasConcept C51724486 @default.
- W2495993102 hasConcept C55493867 @default.
- W2495993102 hasConcept C73826308 @default.
- W2495993102 hasConcept C86803240 @default.
- W2495993102 hasConceptScore W2495993102C134121241 @default.
- W2495993102 hasConceptScore W2495993102C159654299 @default.
- W2495993102 hasConceptScore W2495993102C178790620 @default.
- W2495993102 hasConceptScore W2495993102C179247698 @default.
- W2495993102 hasConceptScore W2495993102C181199279 @default.
- W2495993102 hasConceptScore W2495993102C184651966 @default.
- W2495993102 hasConceptScore W2495993102C185592680 @default.
- W2495993102 hasConceptScore W2495993102C191897082 @default.
- W2495993102 hasConceptScore W2495993102C192562407 @default.
- W2495993102 hasConceptScore W2495993102C203014093 @default.
- W2495993102 hasConceptScore W2495993102C2777468819 @default.
- W2495993102 hasConceptScore W2495993102C2779000548 @default.
- W2495993102 hasConceptScore W2495993102C43617362 @default.
- W2495993102 hasConceptScore W2495993102C515207424 @default.
- W2495993102 hasConceptScore W2495993102C51724486 @default.
- W2495993102 hasConceptScore W2495993102C55493867 @default.
- W2495993102 hasConceptScore W2495993102C73826308 @default.
- W2495993102 hasConceptScore W2495993102C86803240 @default.
- W2495993102 hasLocation W24959931021 @default.
- W2495993102 hasOpenAccess W2495993102 @default.
- W2495993102 hasPrimaryLocation W24959931021 @default.
- W2495993102 hasRelatedWork W1932426075 @default.
- W2495993102 hasRelatedWork W1982820380 @default.
- W2495993102 hasRelatedWork W2016775291 @default.
- W2495993102 hasRelatedWork W2050728149 @default.
- W2495993102 hasRelatedWork W2068632937 @default.
- W2495993102 hasRelatedWork W2072063683 @default.
- W2495993102 hasRelatedWork W2073539346 @default.
- W2495993102 hasRelatedWork W2383013826 @default.
- W2495993102 hasRelatedWork W311734155 @default.
- W2495993102 hasRelatedWork W4231763748 @default.
- W2495993102 isParatext "false" @default.
- W2495993102 isRetracted "false" @default.
- W2495993102 magId "2495993102" @default.
- W2495993102 workType "book-chapter" @default.