FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2496008900> ?p ?o ?g. }

• W2496008900 endingPage "519" @default.
• W2496008900 startingPage "513" @default.
• W2496008900 abstract "The hypoxic environment around the lens is important for maintaining lens transparency. Lens epithelial cells (LECs) play a key role in lens metabolism. We measured oxygen consumption to assess the role of human LECs in maintaining hypoxia around the lens, as well as the impact of systemic and ocular diagnosis on these cells. Baseline cellular respiration was measured in rabbit LECs (NN1003A), canine kidney epithelial cells (MDCK), trabecular meshwork cells (TM-5), and bovine corneal endothelial cells (CCEE) using a XF96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA), which measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in vitro. Following informed written consent, lens capsule epithelial cells were obtained from patients during cataract surgery and were divided into small explants in 96-well plates. Capsules were removed when LECs became confluent. OCR was normalized to the number of cells per well using rabbit LECs as a standard. The effect of patient age, sex, race, and presence of diabetes or glaucoma on oxygen consumption was assessed by using the Mann-Whitney U test and multivariate regression analysis. Primary LECs were obtained from 69 patients. The OCR from donors aged 70 and over was lower than that of those under 70 years (2.21±1.037 vs. 2.86±1.383 fmol/min/cell; p<0.05). Diabetic patients had lower OCR than non-diabetic patients (2.02±0.911 vs. 2.79±1.332 fmol/min/cell; p<0.05), and glaucoma patients had lower OCR than non-glaucoma patients (2.27±1.19 vs. 2.83±1.286 fmol/min/cell; p<0.05). Multivariate regression analysis confirmed that donors aged 70 and over (p<0.05), diabetic patients (p<0.01), and glaucoma patients (p<0.05) had significantly lower OCR, independent of other variables. Gender and race had no significant effect on OCR. The lower oxygen consumption rate of human LECs in older donors and patients with diabetes or glaucoma could contribute to cataract development. Diabetes and glaucoma are particularly important factors associated with decreased OCR, independent of age. Ongoing studies are examining pO2 at the anterior surface of the lens in vivo and oxygen consumption in the patient's LECs." @default.
• W2496008900 created "2016-08-23" @default.
• W2496008900 creator A5014745455 @default.
• W2496008900 creator A5022666933 @default.
• W2496008900 creator A5025103490 @default.
• W2496008900 creator A5048944307 @default.
• W2496008900 creator A5050066810 @default.
• W2496008900 creator A5059866147 @default.
• W2496008900 creator A5068731692 @default.
• W2496008900 creator A5069375705 @default.
• W2496008900 date "2016-08-01" @default.
• W2496008900 modified "2023-10-11" @default.
• W2496008900 title "Mitochondrial oxygen metabolism in primary human lens epithelial cells: Association with age, diabetes and glaucoma" @default.
• W2496008900 cites W1529592271 @default.
• W2496008900 cites W1594562213 @default.
• W2496008900 cites W1936380547 @default.
• W2496008900 cites W1964736306 @default.
• W2496008900 cites W1969856624 @default.
• W2496008900 cites W1973075403 @default.
• W2496008900 cites W1974991148 @default.
• W2496008900 cites W1993660418 @default.
• W2496008900 cites W2000896555 @default.
• W2496008900 cites W2001016880 @default.
• W2496008900 cites W2006741442 @default.
• W2496008900 cites W2006754280 @default.
• W2496008900 cites W2011520923 @default.
• W2496008900 cites W2011892782 @default.
• W2496008900 cites W2012885846 @default.
• W2496008900 cites W2024087110 @default.
• W2496008900 cites W2033247533 @default.
• W2496008900 cites W2040788720 @default.
• W2496008900 cites W2046344578 @default.
• W2496008900 cites W2057689939 @default.
• W2496008900 cites W2058524214 @default.
• W2496008900 cites W2062926254 @default.
• W2496008900 cites W2066766320 @default.
• W2496008900 cites W2071490661 @default.
• W2496008900 cites W2082937294 @default.
• W2496008900 cites W2085050051 @default.
• W2496008900 cites W2085396235 @default.
• W2496008900 cites W2087124521 @default.
• W2496008900 cites W2092895723 @default.
• W2496008900 cites W2093091723 @default.
• W2496008900 cites W2093672336 @default.
• W2496008900 cites W2096623583 @default.
• W2496008900 cites W2104141601 @default.
• W2496008900 cites W2112299930 @default.
• W2496008900 cites W2114620690 @default.
• W2496008900 cites W2125607450 @default.
• W2496008900 cites W2127791137 @default.
• W2496008900 cites W2137843460 @default.
• W2496008900 cites W2138200625 @default.
• W2496008900 cites W2138751275 @default.
• W2496008900 cites W2144790993 @default.
• W2496008900 cites W2146861549 @default.
• W2496008900 cites W2151040450 @default.
• W2496008900 cites W2151767757 @default.
• W2496008900 cites W2157512814 @default.
• W2496008900 cites W2158182196 @default.
• W2496008900 cites W2160916653 @default.
• W2496008900 cites W2170758738 @default.
• W2496008900 cites W2410084468 @default.
• W2496008900 cites W2581760821 @default.
• W2496008900 cites W4242840524 @default.
• W2496008900 cites W4318971849 @default.
• W2496008900 cites W837933935 @default.
• W2496008900 cites W99534246 @default.
• W2496008900 doi "https://doi.org/10.1016/j.freeradbiomed.2016.07.016" @default.
• W2496008900 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4996752" @default.
• W2496008900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27445101" @default.
• W2496008900 hasPublicationYear "2016" @default.
• W2496008900 type Work @default.
• W2496008900 sameAs 2496008900 @default.
• W2496008900 citedByCount "25" @default.
• W2496008900 countsByYear W24960089002017 @default.
• W2496008900 countsByYear W24960089002018 @default.
• W2496008900 countsByYear W24960089002019 @default.
• W2496008900 countsByYear W24960089002020 @default.
• W2496008900 countsByYear W24960089002021 @default.
• W2496008900 countsByYear W24960089002022 @default.
• W2496008900 countsByYear W24960089002023 @default.
• W2496008900 crossrefType "journal-article" @default.
• W2496008900 hasAuthorship W2496008900A5014745455 @default.
• W2496008900 hasAuthorship W2496008900A5022666933 @default.
• W2496008900 hasAuthorship W2496008900A5025103490 @default.
• W2496008900 hasAuthorship W2496008900A5048944307 @default.
• W2496008900 hasAuthorship W2496008900A5050066810 @default.
• W2496008900 hasAuthorship W2496008900A5059866147 @default.
• W2496008900 hasAuthorship W2496008900A5068731692 @default.
• W2496008900 hasAuthorship W2496008900A5069375705 @default.
• W2496008900 hasBestOaLocation W24960089002 @default.
• W2496008900 hasConcept C118487528 @default.
• W2496008900 hasConcept C151730666 @default.
• W2496008900 hasConcept C15336307 @default.
• W2496008900 hasConcept C16685009 @default.
• W2496008900 hasConcept C2776882836 @default.
• W2496008900 hasConcept C2778527774 @default.
• W2496008900 hasConcept C2781121420 @default.

• next