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- W2496312599 abstract "Dehydriny jsou stresovĂŠ proteiny souvisejĂcĂ s tolerancĂ k mrazu a suchu. CĂlem naĹĄĂ prĂĄce bylo studium polymorfismu dehydrinovĂ˝ch genĹŻ (Dhn) v souvislosti s mrazuvzdornostĂ u jeÄmene. Pro studium dĂŠlkovĂŠho polymorfismu u 30 odrĹŻd jsme pouĹžili specifickĂŠ primery pro sekvence genĹŻ Dhn4 a Dhn7. PCR produkty vykĂĄzaly dvÄ odliĹĄnĂŠ matrice; prvnĂ (sekvence typu O) byla specifickĂĄ pro ĹĄestiĹadĂŠ ozimĂŠ a pĹechodnĂŠ odrĹŻdy s vysokou tolerancĂ k mrazu; typickĂ˝m pĹedstavitelem byla odrĹŻda Okal. DruhĂ˝ typ (A sekvence) byla zjiĹĄtÄna jen u dvouĹadĂ˝ch ozimĂ˝ch a u jarnĂch odrĹŻd (napĹ. u odrĹŻdy Akcent) a souvisela s niŞťà mrazuvzdornostĂ. DĂŠlka hlavnĂch polymorfnĂch produktĹŻ odpovĂdala rĹŻznĂ˝m alelĂĄm genĹŻ Dhn4 a Dhn7. RozdĂl v uvedenĂ˝ch dĂŠlkĂĄch byl zpĹŻsoben souÄasnou pĹĂtomnostĂ dvou specifickĂ˝ch mutacĂ, tj. inzercĂ 6 bp v Dhn4 a delecĂ 30 bp v Dhn7 u odrĹŻd typu O." @default.
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- W2496312599 date "2010-02-01" @default.
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- W2496312599 title "Dehydrins as stress proteins related to frost tolerance of barley." @default.
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- W2496312599 doi "https://doi.org/10.18832/kp2010010" @default.
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