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- W2496706536 abstract "Publisher Summary This chapter describes a rabbit model of bacterial endocarditis, in which the white New Zealand female rabbits weighing 2–2.5 kg are the most commonly used. The rabbits are mostly anesthetized by intramuscular injection with ketamine hydrochloride (15 mg/kg). This protocol produces transient anesthesia lasting 15–20 minutes, which corresponds to the duration necessary for surgery. Two main routes of administration have been used for infection. In the model proposed by Garrison and Freedman, the catheter itself was filled with a suspension of Staphylococcus aureus before insertion and the vegetations became infected. However, these experiments were not reproducing the clinical situation, in which cardiac valves are colonized by circulating microorganisms. Durack and Beeson first described the production of bacterial endocarditis after a single intravenous injection of bacteria through a peripheral ear vein. This technique is currently the most frequently used and is very easy to perform. In this technique, after shaving the external part of the ear and producing vasodilatation by applying ethyl alcohol, the inoculum is injected into the vein with a fine needle. The infected rabbits develop clinical and biological manifestations that are very close to those observed in humans with infective endocarditis, such as fever, weight loss, anemia, positive blood cultures, peripheral emboli, and secondary septic localizations. Spontaneous mortality may rapidly occur, particularly when infective bacteria are highly virulent. However, the most reliable parameter to monitor infection is the count of colony forming units (cfu) of bacteria per gram of vegetations." @default.
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- W2496706536 date "1999-01-01" @default.
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- W2496706536 title "Rabbit Model of Bacterial Endocarditis" @default.
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- W2496706536 doi "https://doi.org/10.1016/b978-012775390-4/50211-6" @default.
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