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- W2496918778 abstract "Olfactory ensheathing cellsOlfactory ensheathing cells (OECsOECs ) are a specialized type of gliaglia that supports axonal regenerationregeneration of olfactory neuronsolfactory neurons . OECs derived from olfactory mucosaolfactory mucosa represent a potential candidate for therapeutic use in neurological disordersneurological disorders , like amyotrophic lateral sclerosisamyotrophic lateral sclerosis (ALSALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatalneonatal mousemouse olfactory mucosamucosa by means of an anti-Thy 1.2Thy 1.2 antibodyantibody leading to a specific cytotoxiccytotoxic lysislysis of contaminating fibroblastsfibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100S100 and p75 neurotrophin receptorsp75 neurotrophin receptor (p75NTRp75NTR ), are plated onto lamininlaminin -coated coverslipscoverslips for immunofluorescenceimmunofluorescence characterizationcharacterization , or Petri dishesPetri dishes for expansion and further investigations." @default.
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- W2496918778 date "2015-01-01" @default.
- W2496918778 modified "2023-10-16" @default.
- W2496918778 title "Purification and Characterization of Mouse Olfactory Ensheathing Cells and Their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS)" @default.
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- W2496918778 doi "https://doi.org/10.1007/978-1-4939-2313-7_11" @default.
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