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- W2497615237 abstract "A new easily scalable approach to the recovery of biologically active oligosaccharides from milk has been developed which relies on the combination of en- zymatic treatment of defatted milk using b-galactosidase and nanofiltration. It was shown that enzymatic hydroly- sis of lactose significantly improves the efficiency and selectivity of membrane-based separations. With the best membrane, as much as 6.7 g of oligosaccharides (containing very little contaminating lactose) could be obtained from one liter of defatted human milk in just four nanofiltration cycles. The human milk oligosaccha- rides recovered by this method were shown to inhibit binding of intimin, an adhesion molecule of enteropatho- genic Escherichia coli, to epithelial cells in vitro. No sig- nificant difference in the oligosaccharide profile between samples prepared by this method and conventional gel- permeation chromatography was found. The developed approach is also suitable for the recovery of substantial quantities of tri- and tetra-saccharides from caprine milk. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 461-467, 2000." @default.
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- W2497615237 date "2000-01-01" @default.
- W2497615237 modified "2023-09-23" @default.
- W2497615237 title "COMMUNICATION TO THE EDITOR A Novel Approach to the Recovery of Biologically Active Oligosaccharides from Milk Using a Combination of Enzymatic Treatment and Nanofiltration" @default.
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