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- W2497848463 abstract "Barley (Hordeum vulgare L. cv. Golf) was grown in solution culture with controlled nitrogen availability in order to study ammonia emission from leaves. Ammonia emission measured in cuvettes connected to an automatic NH 3 monitor was close to zero for nitrate grown plants but increased to 0.88 nmol m -2 s -1 after 3 days of ammonium (2 mM) supply. Ammonium in the root medium also increased tissue ammonium contents and xylem sap ammonium concentration. Inhibition of glutamine synthetase (GS, EC 6.3.1.2.) by 0.5 mM methionine sulfoximine (MSO) caused after 2-3 h ammonia emission to increase dramatically (5-10 nmol NH 3 m -2 s -1 ) in both nitrate and ammonium grown plants. After a dark period the ammonia emission was further increased and had reached 30-50 nmol NH 3 m -2 s -1 after 24 h. At the same time both tissue ammonium contents and xylem sap ammonium concentrations increased while glutamine synthetase activities of both root and shoot decreased. Compensation points for ammonia (the air concentration at which no net in- or out-flux of ammonia occurs through stomata) were determined in chamber measurements with air concentrations from 0 to 40 nmol m -2 s -1 of NH 3 . For two cultivars of barley (Golf and Laevigatum) the compensation points decreased from ca. 5 nmol NH 3 mol -1 air during the vegetative growth stage to below 1 nmol NH 3 mol -1 air just after anthesis and then increased again during senescence." @default.
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- W2497848463 date "1996-01-01" @default.
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- W2497848463 title "Characteristics of ammonia emission from barley plants" @default.
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