FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2498459056> ?p ?o ?g. }

• W2498459056 endingPage "20738" @default.
• W2498459056 startingPage "20729" @default.
• W2498459056 abstract "Caveolae are the primary route for internalization and transendothelial transport of macromolecules, such as insulin and albumin. Caveolae-mediated endocytosis is activated by Src-dependent caveolin-1 (Cav-1) phosphorylation and subsequent recruitment of dynamin-2 and filamin A (FilA), which facilitate vesicle fission and trafficking, respectively. Here, we tested the role of RalA and phospholipase D (PLD) signaling in the regulation of caveolae-mediated endocytosis and trafficking. The addition of albumin to human lung microvascular endothelial cells induced the activation of RalA within minutes, and siRNA-mediated down-regulation of RalA abolished fluorescent BSA uptake. Co-immunoprecipitation studies revealed that albumin induced the association between RalA, Cav-1, and FilA; however, RalA knockdown with siRNA did not affect FilA recruitment to Cav-1, suggesting that RalA was not required for FilA and Cav-1 complex formation. Rather, RalA probably facilitates caveolae-mediated endocytosis by activating downstream effectors. PLD2 was shown to be activated by RalA, and inhibition of PLD2 abolished Alexa-488-BSA uptake, indicating that phosphatidic acid (PA) generated by PLD2 may facilitate caveolae-mediated endocytosis. Furthermore, using a PA biosensor, GFP-PASS, we observed that BSA induced an increase in PA co-localization with Cav-1-RFP, which could be blocked by a dominant negative PLD2 mutant. Total internal reflection fluorescence microscopy studies of Cav-1-RFP also showed that fusion of caveolae with the basal plasma membrane was dependent on PLD2 activity. Thus, our results suggest that the small GTPase RalA plays an important role in promoting invagination and trafficking of caveolae, not by potentiating the association between Cav-1 and FilA but by stimulating PLD2-mediated generation of phosphatidic acid. Caveolae are the primary route for internalization and transendothelial transport of macromolecules, such as insulin and albumin. Caveolae-mediated endocytosis is activated by Src-dependent caveolin-1 (Cav-1) phosphorylation and subsequent recruitment of dynamin-2 and filamin A (FilA), which facilitate vesicle fission and trafficking, respectively. Here, we tested the role of RalA and phospholipase D (PLD) signaling in the regulation of caveolae-mediated endocytosis and trafficking. The addition of albumin to human lung microvascular endothelial cells induced the activation of RalA within minutes, and siRNA-mediated down-regulation of RalA abolished fluorescent BSA uptake. Co-immunoprecipitation studies revealed that albumin induced the association between RalA, Cav-1, and FilA; however, RalA knockdown with siRNA did not affect FilA recruitment to Cav-1, suggesting that RalA was not required for FilA and Cav-1 complex formation. Rather, RalA probably facilitates caveolae-mediated endocytosis by activating downstream effectors. PLD2 was shown to be activated by RalA, and inhibition of PLD2 abolished Alexa-488-BSA uptake, indicating that phosphatidic acid (PA) generated by PLD2 may facilitate caveolae-mediated endocytosis. Furthermore, using a PA biosensor, GFP-PASS, we observed that BSA induced an increase in PA co-localization with Cav-1-RFP, which could be blocked by a dominant negative PLD2 mutant. Total internal reflection fluorescence microscopy studies of Cav-1-RFP also showed that fusion of caveolae with the basal plasma membrane was dependent on PLD2 activity. Thus, our results suggest that the small GTPase RalA plays an important role in promoting invagination and trafficking of caveolae, not by potentiating the association between Cav-1 and FilA but by stimulating PLD2-mediated generation of phosphatidic acid." @default.
• W2498459056 created "2016-08-23" @default.
• W2498459056 creator A5025226128 @default.
• W2498459056 creator A5029233987 @default.
• W2498459056 creator A5030639080 @default.
• W2498459056 creator A5035375282 @default.
• W2498459056 creator A5040737368 @default.
• W2498459056 creator A5059262898 @default.
• W2498459056 creator A5065776852 @default.
• W2498459056 creator A5068972249 @default.
• W2498459056 date "2016-09-01" @default.
• W2498459056 modified "2023-10-16" @default.
• W2498459056 title "Phosphatidic Acid Produced by RalA-activated PLD2 Stimulates Caveolae-mediated Endocytosis and Trafficking in Endothelial Cells" @default.
• W2498459056 cites W1549194476 @default.
• W2498459056 cites W1566432282 @default.
• W2498459056 cites W1597709568 @default.
• W2498459056 cites W1855741646 @default.
• W2498459056 cites W1988715014 @default.
• W2498459056 cites W1988873174 @default.
• W2498459056 cites W1989971071 @default.
• W2498459056 cites W1990953109 @default.
• W2498459056 cites W1994736776 @default.
• W2498459056 cites W1996584317 @default.
• W2498459056 cites W2001793483 @default.
• W2498459056 cites W2002594335 @default.
• W2498459056 cites W2004102471 @default.
• W2498459056 cites W2008838494 @default.
• W2498459056 cites W2009018171 @default.
• W2498459056 cites W2014211428 @default.
• W2498459056 cites W2014508855 @default.
• W2498459056 cites W2015752928 @default.
• W2498459056 cites W2026403140 @default.
• W2498459056 cites W2028302114 @default.
• W2498459056 cites W2039165813 @default.
• W2498459056 cites W2040566502 @default.
• W2498459056 cites W2043227433 @default.
• W2498459056 cites W2046209146 @default.
• W2498459056 cites W2049013227 @default.
• W2498459056 cites W2049226881 @default.
• W2498459056 cites W2053598967 @default.
• W2498459056 cites W2060334268 @default.
• W2498459056 cites W2070607055 @default.
• W2498459056 cites W2071946819 @default.
• W2498459056 cites W2073296814 @default.
• W2498459056 cites W2086562476 @default.
• W2498459056 cites W2088125273 @default.
• W2498459056 cites W2092329631 @default.
• W2498459056 cites W2098898583 @default.
• W2498459056 cites W2109110743 @default.
• W2498459056 cites W2118689626 @default.
• W2498459056 cites W2126326200 @default.
• W2498459056 cites W2131291909 @default.
• W2498459056 cites W2145744696 @default.
• W2498459056 cites W2150097452 @default.
• W2498459056 cites W2153742001 @default.
• W2498459056 cites W2157483796 @default.
• W2498459056 cites W2159186465 @default.
• W2498459056 cites W2159891067 @default.
• W2498459056 cites W2160718001 @default.
• W2498459056 cites W2161648012 @default.
• W2498459056 cites W2162316048 @default.
• W2498459056 cites W2170022426 @default.
• W2498459056 cites W2171262890 @default.
• W2498459056 cites W2327605668 @default.
• W2498459056 cites W2387313945 @default.
• W2498459056 doi "https://doi.org/10.1074/jbc.m116.752485" @default.
• W2498459056 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5034062" @default.
• W2498459056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27510034" @default.
• W2498459056 hasPublicationYear "2016" @default.
• W2498459056 type Work @default.
• W2498459056 sameAs 2498459056 @default.
• W2498459056 citedByCount "30" @default.
• W2498459056 countsByYear W24984590562017 @default.
• W2498459056 countsByYear W24984590562018 @default.
• W2498459056 countsByYear W24984590562019 @default.
• W2498459056 countsByYear W24984590562020 @default.
• W2498459056 countsByYear W24984590562021 @default.
• W2498459056 countsByYear W24984590562022 @default.
• W2498459056 crossrefType "journal-article" @default.
• W2498459056 hasAuthorship W2498459056A5025226128 @default.
• W2498459056 hasAuthorship W2498459056A5029233987 @default.
• W2498459056 hasAuthorship W2498459056A5030639080 @default.
• W2498459056 hasAuthorship W2498459056A5035375282 @default.
• W2498459056 hasAuthorship W2498459056A5040737368 @default.
• W2498459056 hasAuthorship W2498459056A5059262898 @default.
• W2498459056 hasAuthorship W2498459056A5065776852 @default.
• W2498459056 hasAuthorship W2498459056A5068972249 @default.
• W2498459056 hasBestOaLocation W24984590561 @default.
• W2498459056 hasConcept C102747710 @default.
• W2498459056 hasConcept C139770010 @default.
• W2498459056 hasConcept C1491633281 @default.
• W2498459056 hasConcept C156407911 @default.
• W2498459056 hasConcept C180373561 @default.
• W2498459056 hasConcept C185592680 @default.
• W2498459056 hasConcept C2776909261 @default.
• W2498459056 hasConcept C2778918659 @default.
• W2498459056 hasConcept C2780298669 @default.
• W2498459056 hasConcept C28005876 @default.

• next