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- W2498906686 abstract "The absolute necessity for maintaining accurate records of experiments performed and the resulting data is indisputable. Electrophoresis gels contain information critical for accurate interpretation of the outcome of a particular experiment. In both analytical and preparative settings, the information obtained by electrophoresis may be invaluable. Gels deteriorate in a relatively short time, however, and the dyes used to stain nucleic acids in gels deteriorate even more rapidly. This mandates archiving the image electronically and/or producing a tangible photographic record, as well as a photographic record of any associated X-ray films generated upon hybridization detection. A variety of systems have gained widespread acceptance and are currently in use to record and analyze the various types of images commonly encountered in a molecular biology setting. These systems fall into two broad categories: digital imaging, in which the image of the gel or X-ray film is analyzed by and stored in a computer and/or on a disk; and the traditional photograph generated on thermal paper or Polaroid film. Useful measurements that are now automated include determination of molecular weight, concentration, relative abundance, integrated optical density (IOD), and image saturation. The computer also provides a means of cataloging stored images, whereas an image analysis system most often consists of a completely self-contained system, such as the fotodyne luminary/FX workstation, which is in constant use in the laboratory for archiving the images." @default.
- W2498906686 created "2016-08-23" @default.
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- W2498906686 date "2010-01-01" @default.
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- W2498906686 title "Photodocumentation and Image Analysis" @default.
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- W2498906686 doi "https://doi.org/10.1016/b978-0-12-374727-3.00010-3" @default.
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