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• W2498989218 abstract "Virtual microfluidics uses hydrogel entrapment to make high-throughput single-cell and single-molecule amplification broadly accessible without the need for special equipment. We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions." @default.
• W2498989218 created "2016-08-23" @default.
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• W2498989218 date "2016-08-01" @default.
• W2498989218 modified "2023-10-18" @default.
• W2498989218 title "Virtual microfluidics for digital quantification and single-cell sequencing" @default.
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• W2498989218 doi "https://doi.org/10.1038/nmeth.3955" @default.
• W2498989218 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5007149" @default.
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