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- W2499202655 endingPage "164" @default.
- W2499202655 startingPage "139" @default.
- W2499202655 abstract "With the current advancements in DNA technology, from cheap synthesis to synthetic biology, it is now possible to design, in a modular and combinatorial fashion, not only the genes that will structure the heterologous metabolic pathways, but also the regulatory elements that will ensure the optimal expression levels of such genes. In the realm of biofuels, such biotechnologies have established a clear path toward successfully engineering microbes with features like high yields, titers, and productivities, which are the main goal in any industrial production. This chapter summarizes outstanding technologies that have shown capability to assemble pathways in a standard, quick, and efficient manner. Combinatorial pathway assembly, a trend that has been enabled by exponential emergence of synthetic biology tools will be discussed in the main body of this chapter. To finalize, we discuss the current challenges revolving around the availability of high-throughput screening techniques and automated processes. These technologies aim to potentiate the assembly of pathways for the production of biofuels and other metabolites of interest." @default.
- W2499202655 created "2016-08-23" @default.
- W2499202655 creator A5052560078 @default.
- W2499202655 creator A5067876418 @default.
- W2499202655 creator A5081293544 @default.
- W2499202655 date "2016-01-01" @default.
- W2499202655 modified "2023-09-27" @default.
- W2499202655 title "Pathway Assembly and Optimization" @default.
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