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- W2499388082 abstract "ABSTRACT Chlamydomonas is the only plant whose chloroplast genome can be studied by recombination analysis. Our previous results show that chloroplast mutations resistant to antibacterial antibiotics define a group of 7 closely linked loci comprising a ribosomal region and cause the chloroplast ribosomes to become drug resistant. Recently we have isolated many chloroplast mutations defective in photosynthesis using the thymidine analog 5-fluorodeoxyuridine which increases specifically the yield of all known chloroplast mutant phenotypes. The 16 non-photosynthetic mutants characterized so far fall in 9 recombinationally distinct loci, one of which has now been mapped some distance from the ribosomal region. Mutations at 2 of these non-photosynthetic loci cause defects in chloroplast ribosome assembly; mutations at 3 loci result in the loss of chlorophyll-protein complex 1 from thylakoid membranes; and mutations at 3 other loci cause the disappearance of at least 2 of the 4 CF 1 -ATPase polypeptides from the thylakoids. To orient our genetic map of the chloroplast genome with respect to the physical map made with restriction endonucleases, we are screening our collection of chloroplast mutants for deletions, insertions or other alterations in the chloroplast genome. Once such physical markers have been found, we will map them genetically with respect to mutants at the 16 chloroplast loci identified by recombination. Thus far we have observed several differences in restriction digest patterns of chloroplast DNA. One non-photosynthetic mutant stock contains deletions in two separate EcoRI fragments. Preliminary results indicate that this stock cotransmits both deletions in crosses with the maternal inheritance pattern characteristic of all known chloroplast mutations." @default.
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- W2499388082 date "1979-01-01" @default.
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- W2499388082 title "GENETIC ANALYSIS OF CHLOROPLAST DNA FUNCTION IN CHLAMYDOMONAS11This work was supported by NIH grant GM-19427; Anna Fuller Foundation and NIH postdoctoral fellowship GM-05822 to DMG; NIH postdoctoral fellowship GM-05396 to EAW and NIH predoctoral traineeship GM-02007 to HSS." @default.
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- W2499388082 doi "https://doi.org/10.1016/b978-0-12-198780-0.50011-8" @default.
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