Matches in SemOpenAlex for { <https://semopenalex.org/work/W2499681389> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2499681389 abstract "The in vivo formation of covalent aflatoxin B1 (AFB1)-DNA adducts within the rRNA gene sequences of nu- clear DNA has been studied in AFBl-treated rats. Liver nucle- ar DNA, enriched in ribosomal DNA (rDNA) by one round of cesium salt density gradient centrifugation, was treated under buffered alkaline conditions to convert unstable AFB1-N7-gua- nine adducts to stable AFBi-formamidopyrimidine deriva- tives. The alkali-treated DNA was hybridized to 18S and 28S rRNA in 70% formamide buffer to form rRNA-rDNA hybrids. These hybrids were separated from the bulk of nuclear DNA by two rounds of centrifugation in CsCI, and the level of AFB1 adduction to rDNA versus total nuclear DNA was compared as a function of dose 2 hr after AFB1 administration. Over an 8- fold dose range (0.25-2.0 mg of AFB1 per kg of body weight), rDNA contained 4- to 5-fold more AFBi residues than nuclear DNA, indicating that rDNA is preferentially accessible to car- cinogen modification in vivo. While aflatoxin B1 forms adducts with DNA principally at guanine residues, the guanine enrich- ment of rDNA was insufficient to explain the magnitude of ob- served preferential AFB1 modification of rDNA. These results support the hypothesis that rDNA regions are preferentially accessible to carcinogen modification because of the diffuse conformation maintained within transcribed genes. This ex- perimental approach permits the quantitative description of carcinogen modification within a defined gene sequence; fur- ther refinement of this approach may be useful in defining the precise relationships between covalent chemical-DNA interac- tions and the alterations in gene expression that result." @default.
- W2499681389 created "2016-08-23" @default.
- W2499681389 creator A5064103723 @default.
- W2499681389 creator A5087964229 @default.
- W2499681389 date "2016-01-01" @default.
- W2499681389 modified "2023-09-27" @default.
- W2499681389 title "Quantitation of aflatoxin B1 adduct gene sequences of rat liver DNA (carcinogen-nucleic acid interactions/DNA-RNA hybridization)" @default.
- W2499681389 cites W2087873413 @default.
- W2499681389 hasPublicationYear "2016" @default.
- W2499681389 type Work @default.
- W2499681389 sameAs 2499681389 @default.
- W2499681389 citedByCount "0" @default.
- W2499681389 crossrefType "journal-article" @default.
- W2499681389 hasAuthorship W2499681389A5064103723 @default.
- W2499681389 hasAuthorship W2499681389A5087964229 @default.
- W2499681389 hasConcept C104317684 @default.
- W2499681389 hasConcept C115960442 @default.
- W2499681389 hasConcept C153911025 @default.
- W2499681389 hasConcept C185592680 @default.
- W2499681389 hasConcept C24107716 @default.
- W2499681389 hasConcept C24586158 @default.
- W2499681389 hasConcept C2778301229 @default.
- W2499681389 hasConcept C512185932 @default.
- W2499681389 hasConcept C54871578 @default.
- W2499681389 hasConcept C552990157 @default.
- W2499681389 hasConcept C55493867 @default.
- W2499681389 hasConcept C67705224 @default.
- W2499681389 hasConcept C67905577 @default.
- W2499681389 hasConcept C86803240 @default.
- W2499681389 hasConceptScore W2499681389C104317684 @default.
- W2499681389 hasConceptScore W2499681389C115960442 @default.
- W2499681389 hasConceptScore W2499681389C153911025 @default.
- W2499681389 hasConceptScore W2499681389C185592680 @default.
- W2499681389 hasConceptScore W2499681389C24107716 @default.
- W2499681389 hasConceptScore W2499681389C24586158 @default.
- W2499681389 hasConceptScore W2499681389C2778301229 @default.
- W2499681389 hasConceptScore W2499681389C512185932 @default.
- W2499681389 hasConceptScore W2499681389C54871578 @default.
- W2499681389 hasConceptScore W2499681389C552990157 @default.
- W2499681389 hasConceptScore W2499681389C55493867 @default.
- W2499681389 hasConceptScore W2499681389C67705224 @default.
- W2499681389 hasConceptScore W2499681389C67905577 @default.
- W2499681389 hasConceptScore W2499681389C86803240 @default.
- W2499681389 hasLocation W24996813891 @default.
- W2499681389 hasOpenAccess W2499681389 @default.
- W2499681389 hasPrimaryLocation W24996813891 @default.
- W2499681389 hasRelatedWork W1493159717 @default.
- W2499681389 hasRelatedWork W1562454145 @default.
- W2499681389 hasRelatedWork W1581090731 @default.
- W2499681389 hasRelatedWork W1967530571 @default.
- W2499681389 hasRelatedWork W1968071903 @default.
- W2499681389 hasRelatedWork W1976044291 @default.
- W2499681389 hasRelatedWork W1985881584 @default.
- W2499681389 hasRelatedWork W1994965840 @default.
- W2499681389 hasRelatedWork W1998708818 @default.
- W2499681389 hasRelatedWork W2036330970 @default.
- W2499681389 hasRelatedWork W2039692985 @default.
- W2499681389 hasRelatedWork W2055488152 @default.
- W2499681389 hasRelatedWork W2059752413 @default.
- W2499681389 hasRelatedWork W2078461939 @default.
- W2499681389 hasRelatedWork W2118079495 @default.
- W2499681389 hasRelatedWork W2141879101 @default.
- W2499681389 hasRelatedWork W2149189393 @default.
- W2499681389 hasRelatedWork W2196945965 @default.
- W2499681389 hasRelatedWork W1564880602 @default.
- W2499681389 hasRelatedWork W2024478616 @default.
- W2499681389 isParatext "false" @default.
- W2499681389 isRetracted "false" @default.
- W2499681389 magId "2499681389" @default.
- W2499681389 workType "article" @default.