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• W2499790151 abstract "The content of the Alternaria toxin tenuazonic acid and its isomer allo-tenuazonic acid was quantitated in urine of a German cohort (n = 48) using a newly developed and successfully validated solid phase extraction based stable isotope dilution HPLC-MS3 method. Tenuazonic acid was detected in all of the samples and quantifiable in 97.9% of these samples in a range of 0.16–44.4 ng/mL (average = 6.58 ng/mL) or 0.07–63.8 ng/mg creatinine (average = 8.13 ng/mg creatinine). allo-Tenuazonic acid was for the first time detected in human urine (95.8% of the samples positive) and quantitated in 68.8% of the samples in a range of 0.11–5.72 ng/mL (average = 1.25 ng/mL) or 0.08–10.1 ng/mg creatinine (average = 1.52 ng/mg creatinine), representing 3.40–25.0% of the sum of both isomers (average = 12.4%). Food-frequency questionnaires were used to document food consumption of study participants to correlate mycotoxin exposure to nutritional habits. Although no statistically significant correlation between consumption of a specific food and urinary excretion of tenuazonic acid could be determined, a trend regarding elevated intake of cereal products and higher excretion of tenuazonic acid was evident. On the basis of these results, a provisional mean daily intake (PDI) for both tenuazonic acid and allo-tenuazonic acid was calculated, being 0.183 and 0.025 μg/kg body weight, respectively. A combined mean PDI for both isomers amounts to 0.208 μg/kg body weight with the highest individual PDI for one of the participants (1.582 μg/kg body weight) slightly exceeding the threshold of toxicological concern assumed for tenuazonic acid by the European Food Safety Authority of 1.500 μg/kg body weight. This is the first study to investigate the tenuazonic acid content in human urine of a larger sample cohort enabling the calculation of PDIs for tenuazonic acid and allo-tenuazonic acid." @default.
• W2499790151 created "2016-08-23" @default.
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• W2499790151 date "2016-08-22" @default.
• W2499790151 modified "2023-09-27" @default.
• W2499790151 title "Determination of Exposure to the <i>Alternaria</i> Mycotoxin Tenuazonic Acid and Its Isomer <i>allo</i>-Tenuazonic Acid in a German Population by Stable Isotope Dilution HPLC-MS³" @default.
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• W2499790151 doi "https://doi.org/10.1021/acs.jafc.6b02735" @default.
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