Matches in SemOpenAlex for { <https://semopenalex.org/work/W2499837078> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2499837078 endingPage "968" @default.
- W2499837078 startingPage "964" @default.
- W2499837078 abstract "An indirect enzyme-linked immunosorbent assay (ELISA) which can detect 0.2 to 1 ng of T-2 toxin per ml in urine, serum and milk was developed. T-2 hemisuccinate was conjugated to polylysine which was then coated to a microtiter plate and incubated with rabbit anti-T-2 antibody and sample extract. The amount of anti-T-2 antibody bound to the plate was then determined by reaction with goat anti-rabbit IgG-peroxidase complex and by subsequent reaction with the substrate. Samples spiked with T-2 toxin were subjected to a simple cleanup procedure by passing them through a reversed-phase Sep-Pak catridge (C18). The recoveries of tritiated T-2 toxin added to the urine, serum and milk samples were between 71 to 90% after the cleanup step. In the ELISA, significant interference was observed when more than 5 μl of sample, without cleanup treatment, were used in each analysis. After cleanup, extracts equivalent to 50 μl of serum, urine or milk per well did not significantly interfere with the assay. The recoveries of T-2 toxin added to serum (1 to 10 ng/ml), urine (0.2 to 10 ng/ml) and milk (0.2 to 10 ng/ml) after cleanup treatment as determined by the indirect ELISA were found to be 51 to 82%, 73 to 82% and 80 to 83%, respectively." @default.
- W2499837078 created "2016-08-23" @default.
- W2499837078 creator A5054562455 @default.
- W2499837078 creator A5062671055 @default.
- W2499837078 creator A5080710084 @default.
- W2499837078 date "1984-12-01" @default.
- W2499837078 modified "2023-09-26" @default.
- W2499837078 title "An Indirect Enzyme-Linked Immunosorbent Assay for T-2 Toxin in Biological Fluids" @default.
- W2499837078 doi "https://doi.org/10.4315/0362-028x-47.12.964" @default.
- W2499837078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30934396" @default.
- W2499837078 hasPublicationYear "1984" @default.
- W2499837078 type Work @default.
- W2499837078 sameAs 2499837078 @default.
- W2499837078 citedByCount "26" @default.
- W2499837078 countsByYear W24998370782016 @default.
- W2499837078 countsByYear W24998370782018 @default.
- W2499837078 countsByYear W24998370782022 @default.
- W2499837078 crossrefType "journal-article" @default.
- W2499837078 hasAuthorship W2499837078A5054562455 @default.
- W2499837078 hasAuthorship W2499837078A5062671055 @default.
- W2499837078 hasAuthorship W2499837078A5080710084 @default.
- W2499837078 hasBestOaLocation W24998370781 @default.
- W2499837078 hasConcept C159654299 @default.
- W2499837078 hasConcept C181199279 @default.
- W2499837078 hasConcept C185592680 @default.
- W2499837078 hasConcept C203014093 @default.
- W2499837078 hasConcept C2777367657 @default.
- W2499837078 hasConcept C2777676563 @default.
- W2499837078 hasConcept C2780026642 @default.
- W2499837078 hasConcept C2780420688 @default.
- W2499837078 hasConcept C43617362 @default.
- W2499837078 hasConcept C55493867 @default.
- W2499837078 hasConcept C86803240 @default.
- W2499837078 hasConceptScore W2499837078C159654299 @default.
- W2499837078 hasConceptScore W2499837078C181199279 @default.
- W2499837078 hasConceptScore W2499837078C185592680 @default.
- W2499837078 hasConceptScore W2499837078C203014093 @default.
- W2499837078 hasConceptScore W2499837078C2777367657 @default.
- W2499837078 hasConceptScore W2499837078C2777676563 @default.
- W2499837078 hasConceptScore W2499837078C2780026642 @default.
- W2499837078 hasConceptScore W2499837078C2780420688 @default.
- W2499837078 hasConceptScore W2499837078C43617362 @default.
- W2499837078 hasConceptScore W2499837078C55493867 @default.
- W2499837078 hasConceptScore W2499837078C86803240 @default.
- W2499837078 hasIssue "12" @default.
- W2499837078 hasLocation W24998370781 @default.
- W2499837078 hasLocation W24998370782 @default.
- W2499837078 hasOpenAccess W2499837078 @default.
- W2499837078 hasPrimaryLocation W24998370781 @default.
- W2499837078 hasRelatedWork W1551691785 @default.
- W2499837078 hasRelatedWork W1998361048 @default.
- W2499837078 hasRelatedWork W2024219604 @default.
- W2499837078 hasRelatedWork W2035747744 @default.
- W2499837078 hasRelatedWork W2037307292 @default.
- W2499837078 hasRelatedWork W2042826848 @default.
- W2499837078 hasRelatedWork W2084112768 @default.
- W2499837078 hasRelatedWork W210546365 @default.
- W2499837078 hasRelatedWork W2107834236 @default.
- W2499837078 hasRelatedWork W2119622678 @default.
- W2499837078 hasVolume "47" @default.
- W2499837078 isParatext "false" @default.
- W2499837078 isRetracted "false" @default.
- W2499837078 magId "2499837078" @default.
- W2499837078 workType "article" @default.