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• W2500114518 abstract "This chapter uses cytochrome P450 1A1 (CYP1A1) as a model “xenobiotic inducible” gene and the aryl hydrocarbon receptor (AHR) as a regulator of this gene product to illustrate how studies may be performed to understand the relationships between the regulator protein and its target genes. The methodology described was developed to study transcriptional activation. Because transcriptional repression occurs nearly as frequently as activation, the assays described may require modifications to accommodate both mechanisms. Approaches typically used to determine whether changes in gene expression induced by a particular drug or xenobiotic are a result of an increase in gene transcription mediated by specific protein/DNA interactions are described. These approaches and assays include the use of inhibitors of transcription and translation, nuclear runoffs, promoter analysis, and reporter assays to determine the role of specific DNA sequences, determination of specific DNA–protein interactions both in vitro and in vivo using electrophoretic gel mobility shift assays, UV-crosslinking, Southwestern blotting, yeast one-hybrid screening, DNA footprinting, site affinity amplification binding, and the chromatin immunoprecipitation (ChIP) assay, and studies to determine functional roles for specific transcription factors." @default.
• W2500114518 created "2016-08-23" @default.
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• W2500114518 date "2009-05-14" @default.
• W2500114518 modified "2023-09-24" @default.
• W2500114518 title "Experimental Approaches for the Study of Cytochrome P450 Gene Regulation" @default.
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• W2500114518 doi "https://doi.org/10.1385/1-59259-832-3:017" @default.
• W2500114518 hasPublicationYear "2009" @default.
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