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- W250021757 abstract "目的观察联合应用白介素(IL)-6和1,25(OH)2D3对大鼠骨髓破骨细胞生成诱导的影响.方法采用4周龄SD大鼠无菌条件下取出股骨,剪去两骺端,以α-MEM培养液将骨髓细胞冲出,然后将细胞悬液接种于预置盖玻片或牙本质片的24孔培养板内,24h后换液.试验分为4组,A组:不加任何诱导因子;B组:加入IL-6(10 U/ml);C组:加入1,25(OH)2D3(1 ×10-8mol/L);D组:加入IL-6(10 U/ml)和1,25(OH)2D3(1×10-8mol/L).每组各6孔,于培养的第7天进行多核细胞计数.结果培养1周左右IL-6与1,25(OH)2D3在体外均可单独诱导骨髓破骨细胞的形成,但D组多核细胞数与B、C组相比差异有显著性(P＜0.05).结论IL-6与1,25(OH)2D3联合作用下对骨髓破骨细胞生成的诱导效果明显高于单因素诱导效果." @default.
- W250021757 created "2016-06-24" @default.
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- W250021757 date "2016-10-30" @default.
- W250021757 modified "2023-10-18" @default.
- W250021757 title "白介素-6与1,25(OH) 2维生素D_3联合作用下大鼠骨髓破骨细胞的体外诱导培养" @default.
- W250021757 hasPublicationYear "2016" @default.
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