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• W2500480068 abstract "Production of mycophenolic acid (MPA) by submerged fermentation using the microfungus Penicillium brevicompactum MTCC 8010 is reported here. Screening experiments were used to identify: the suitable media composition; the optimal initial pH; and the optimal incubation temperature to maximize the production of MPA in batch cultures. The initial concentrations of the selected sources of carbon (glucose), nitrogen (peptone) and the precursors (methionine, glycine) were then optimized by: (1) one-at-a-time variation of factors; and (2) a central composite design (CCD) of experiments, in a 12-day batch culture at an initial pH of 5.0, an incubation temperature of 25 °C, and an agitation speed of 200 rpm. The medium optimized using the one-at-a-time variation yielded a peak MPA titer of 1232 ± 90 mg/L. The medium optimized by the CCD method yielded a 40% higher MPA titer of 1737 ± 55 mg/L. The latter value was nearly 9-fold greater than the titer achieved prior to optimization." @default.
• W2500480068 created "2016-08-23" @default.
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• W2500480068 date "2016-09-01" @default.
• W2500480068 modified "2023-10-09" @default.
• W2500480068 title "Production of mycophenolic acid by Penicillium brevicompactum—A comparison of two methods of optimization" @default.
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• W2500480068 doi "https://doi.org/10.1016/j.btre.2016.07.003" @default.
• W2500480068 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5042303" @default.
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