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- W2500762899 abstract "Objective The aim of this study was to perform a comparative investigation of several different thawing protocols and to determine an appropriate protocol for thawing whole bovine frozen ovaries. Study design Bovine ovaries were slowly frozen and then thawed by applying different protocols. Ultrastructural change, follicle viability, and the hormone levels of culture supernatant were measured. Results The percentage of morphologically normal primordial follicles and the hormone levels of culture supernatant in group D (two-step, thawing in water at 39 °C) were significantly higher than those in any other group. Moreover, the ultrastructural alteration of oocyte in group D (two-step, thawing in water at 39 °C) was slighter than those in any other group. Conclusions The two-step protocol involving short-term exposure to water at a moderately high temperature (39 °C) proved to be a suitable for thawing bovine whole ovaries." @default.
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- W2500762899 date "2016-09-01" @default.
- W2500762899 modified "2023-09-25" @default.
- W2500762899 title "Cryopreservation of whole bovine ovaries: comparisons of different thawing protocols" @default.
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- W2500762899 doi "https://doi.org/10.1016/j.ejogrb.2016.06.016" @default.
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