FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2502005123> ?p ?o ?g. }

• W2502005123 abstract "A persistently low survival rate and more than 80% of patients experiencing relapse after surgery combine to make pancreatic ductal adenocarcinoma (PDAC) the 4th leading cause of death from cancer. Recent studies showed that the presence of tumor-associated macrophages (TAMs) may support cancer cells in each step of the metastatic cascade: growth at the primary site followed by epithelial-mesenchymal transition (EMT), intravasation into lymph and blood vessels, circulation to a distant site, stoppage due to adhesion or size restriction, extravasation into tissue, and colonization in this secondary organ. However, the role of TAMs and their metabolic profile in tumor progression need further investigation. The objective of our studies was to examine the potential for macrophages to switch from oxidative phosphorylation to aerobic glycolysis (the Warburg effect), similar to what is observed in cancer cells, and how this metabolic conversion may influence their pro-metastatic activity. To address this question we differentiated human peripheral blood monocytes with non tumor- and tumor-conditioned media to obtain macrophages and TAMs, respectively. First, we showed in a live glycolytic stress test that TAMs had a greater glycolytic capacity compared to non tumor-conditioned macrophages. Next, a 3D in-vitro microfluidic extravasation assay consisting of an endothelial monolayer was used to assess the effect of macrophages on PDAC cell extravasation across the endothelial wall into an extracellular matrix-like hydrogel. We observed that the extravasation propensity of PDAC cells was increased by the presence of TAMs but was not significantly affected by non tumor-conditioned macrophages. This suggests that the tumor-conditioned media fostered monocyte differentiation toward pro-metastatic macrophages. To assess if the macrophage metabolic switch toward glycolysis was the key factor promoting the increment in cancer cell extravasation, we treated the TAMs with 2-Deoxyglucose (2-DG), a competitive inhibitor of glycolysis at the level of hexokinase. Indeed, we observed a significant reduction in the rate of PDAC cell extravasation when TAMs were treated with 2-DG compared to untreated TAMs. Our results confirmed that the glycolytic metabolism was involved in modulating pro-tumoural macrophage function. Taken together, our data suggest that the macrophage metabolic pathway is a potential therapeutic target for controlling immune cell contributions to tumor progression and that patient responses to current treatments might be improved by inhibition of macrophage glycolysis. Citation Format: Giulia Adriani, Hweixian L. Penny, Je Lin Sieow, Siew Cheng Wong, Roger D. Kamm. Exploring the role of tumor-conditioned macrophage metabolism on extravasation of pancreatic ductal adenocarcinoma cells. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1578." @default.
• W2502005123 created "2016-08-23" @default.
• W2502005123 creator A5007338010 @default.
• W2502005123 creator A5024288749 @default.
• W2502005123 creator A5034121073 @default.
• W2502005123 creator A5047618524 @default.
• W2502005123 creator A5091486825 @default.
• W2502005123 date "2016-07-15" @default.
• W2502005123 modified "2023-09-26" @default.
• W2502005123 title "Abstract 1578: Exploring the role of tumor-conditioned macrophage metabolism on extravasation of pancreatic ductal adenocarcinoma cells" @default.
• W2502005123 doi "https://doi.org/10.1158/1538-7445.am2016-1578" @default.
• W2502005123 hasPublicationYear "2016" @default.
• W2502005123 type Work @default.
• W2502005123 sameAs 2502005123 @default.
• W2502005123 citedByCount "2" @default.
• W2502005123 countsByYear W25020051232018 @default.
• W2502005123 countsByYear W25020051232019 @default.
• W2502005123 crossrefType "proceedings-article" @default.
• W2502005123 hasAuthorship W2502005123A5007338010 @default.
• W2502005123 hasAuthorship W2502005123A5024288749 @default.
• W2502005123 hasAuthorship W2502005123A5034121073 @default.
• W2502005123 hasAuthorship W2502005123A5047618524 @default.
• W2502005123 hasAuthorship W2502005123A5091486825 @default.
• W2502005123 hasConcept C121608353 @default.
• W2502005123 hasConcept C126322002 @default.
• W2502005123 hasConcept C142724271 @default.
• W2502005123 hasConcept C185592680 @default.
• W2502005123 hasConcept C20251656 @default.
• W2502005123 hasConcept C202751555 @default.
• W2502005123 hasConcept C2776107976 @default.
• W2502005123 hasConcept C2778050619 @default.
• W2502005123 hasConcept C2779013556 @default.
• W2502005123 hasConcept C2779244956 @default.
• W2502005123 hasConcept C2779256057 @default.
• W2502005123 hasConcept C2779540182 @default.
• W2502005123 hasConcept C2780210213 @default.
• W2502005123 hasConcept C2781182431 @default.
• W2502005123 hasConcept C502942594 @default.
• W2502005123 hasConcept C55493867 @default.
• W2502005123 hasConcept C55885012 @default.
• W2502005123 hasConcept C62231903 @default.
• W2502005123 hasConcept C71924100 @default.
• W2502005123 hasConcept C96232424 @default.
• W2502005123 hasConceptScore W2502005123C121608353 @default.
• W2502005123 hasConceptScore W2502005123C126322002 @default.
• W2502005123 hasConceptScore W2502005123C142724271 @default.
• W2502005123 hasConceptScore W2502005123C185592680 @default.
• W2502005123 hasConceptScore W2502005123C20251656 @default.
• W2502005123 hasConceptScore W2502005123C202751555 @default.
• W2502005123 hasConceptScore W2502005123C2776107976 @default.
• W2502005123 hasConceptScore W2502005123C2778050619 @default.
• W2502005123 hasConceptScore W2502005123C2779013556 @default.
• W2502005123 hasConceptScore W2502005123C2779244956 @default.
• W2502005123 hasConceptScore W2502005123C2779256057 @default.
• W2502005123 hasConceptScore W2502005123C2779540182 @default.
• W2502005123 hasConceptScore W2502005123C2780210213 @default.
• W2502005123 hasConceptScore W2502005123C2781182431 @default.
• W2502005123 hasConceptScore W2502005123C502942594 @default.
• W2502005123 hasConceptScore W2502005123C55493867 @default.
• W2502005123 hasConceptScore W2502005123C55885012 @default.
• W2502005123 hasConceptScore W2502005123C62231903 @default.
• W2502005123 hasConceptScore W2502005123C71924100 @default.
• W2502005123 hasConceptScore W2502005123C96232424 @default.
• W2502005123 hasLocation W25020051231 @default.
• W2502005123 hasOpenAccess W2502005123 @default.
• W2502005123 hasPrimaryLocation W25020051231 @default.
• W2502005123 hasRelatedWork W1963717723 @default.
• W2502005123 hasRelatedWork W2123132717 @default.
• W2502005123 hasRelatedWork W2255926577 @default.
• W2502005123 hasRelatedWork W2265800624 @default.
• W2502005123 hasRelatedWork W2317880258 @default.
• W2502005123 hasRelatedWork W2320599234 @default.
• W2502005123 hasRelatedWork W2335682259 @default.
• W2502005123 hasRelatedWork W2404065001 @default.
• W2502005123 hasRelatedWork W2484459318 @default.
• W2502005123 hasRelatedWork W2624130596 @default.
• W2502005123 hasRelatedWork W2740842623 @default.
• W2502005123 hasRelatedWork W2765136070 @default.
• W2502005123 hasRelatedWork W2793436019 @default.
• W2502005123 hasRelatedWork W2799476413 @default.
• W2502005123 hasRelatedWork W2806317563 @default.
• W2502005123 hasRelatedWork W2910265471 @default.
• W2502005123 hasRelatedWork W2970039515 @default.
• W2502005123 hasRelatedWork W3108912917 @default.
• W2502005123 hasRelatedWork W3134708119 @default.
• W2502005123 hasRelatedWork W3167190056 @default.
• W2502005123 isParatext "false" @default.
• W2502005123 isRetracted "false" @default.
• W2502005123 magId "2502005123" @default.
• W2502005123 workType "article" @default.