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• W250215821 endingPage "e0126009" @default.
• W250215821 startingPage "e0126009" @default.
• W250215821 abstract "Metformin (Met) is a biguanide anti-hyperglycemic agent, which also exerts antiproliferative effects on cancer cells. This drug inhibits the complex I of the mitochondrial electron transport chain inducing a fall in the cell energy charge and leading 5'-AMP-activated protein kinase (AMPK) activation. AMPK is a highly conserved heterotrimeric complex that coordinates metabolic and growth pathways in order to maintain energy homeostasis and cell survival, mainly under nutritional stress conditions, in a Liver Kinase B1 (LKB1)-dependent manner. This work describes for the first time, the in vitro anti-echinococcal effect of Met on Echinococcus granulosus larval stages, as well as the molecular characterization of AMPK (Eg-AMPK) in this parasite of clinical importance. The drug exerted a dose-dependent effect on the viability of both larval stages. Based on this, we proceeded with the identification of the genes encoding for the different subunits of Eg-AMPK. We cloned one gene coding for the catalytic subunit (Eg-ampkɑ) and two genes coding for the regulatory subunits (Eg-ampkβ and Eg-ampkγ), all of them constitutively transcribed in E. granulosus protoscoleces and metacestodes. Their deduced amino acid sequences show all the conserved functional domains, including key amino acids involved in catalytic activity and protein-protein interactions. In protoscoleces, the drug induced the activation of AMPK (Eg-AMPKɑ-P176), possibly as a consequence of cellular energy charge depletion evidenced by assays with the fluorescent indicator JC-1. Met also led to carbohydrate starvation, it increased glucogenolysis and homolactic fermentation, and decreased transcription of intermediary metabolism genes. By in toto immunolocalization assays, we detected Eg-AMPKɑ-P176 expression, both in the nucleus and the cytoplasm of cells as in the larval tegument, the posterior bladder and the calcareous corpuscles of control and Met-treated protoscoleces. Interestingly, expression of Eg-AMPKɑ was observed in the developmental structures during the de-differentiation process from protoscoleces to microcysts. Therefore, the Eg-AMPK expression during the asexual development of E. granulosus, as well as the in vitro synergic therapeutic effects observed in presence of Met plus albendazole sulfoxide (ABZSO), suggest the importance of carrying out chemoprophylactic and clinical efficacy studies combining Met with conventional anti-echinococcal agents to test the potential use of this drug in hydatidosis therapy." @default.
• W250215821 created "2016-06-24" @default.
• W250215821 creator A5013330654 @default.
• W250215821 creator A5055292448 @default.
• W250215821 date "2015-05-12" @default.
• W250215821 modified "2023-10-16" @default.
• W250215821 title "In Vitro Anti-Echinococcal and Metabolic Effects of Metformin Involve Activation of AMP-Activated Protein Kinase in Larval Stages of Echinococcus granulosus" @default.
• W250215821 cites W1582955199 @default.
• W250215821 cites W1862308705 @default.
• W250215821 cites W1964423199 @default.
• W250215821 cites W1965885099 @default.
• W250215821 cites W1972256263 @default.
• W250215821 cites W1979217402 @default.
• W250215821 cites W1980083426 @default.
• W250215821 cites W1980608195 @default.
• W250215821 cites W1985517808 @default.
• W250215821 cites W1986428317 @default.
• W250215821 cites W1986853973 @default.
• W250215821 cites W1986896225 @default.
• W250215821 cites W1987334928 @default.
• W250215821 cites W1987447854 @default.
• W250215821 cites W1988371384 @default.
• W250215821 cites W1990679898 @default.
• W250215821 cites W1991800829 @default.
• W250215821 cites W1995069275 @default.
• W250215821 cites W1996593753 @default.
• W250215821 cites W1997003352 @default.
• W250215821 cites W1998596984 @default.
• W250215821 cites W1998771362 @default.
• W250215821 cites W2000047954 @default.
• W250215821 cites W2002068909 @default.
• W250215821 cites W2009146697 @default.
• W250215821 cites W2009263255 @default.
• W250215821 cites W2010238890 @default.
• W250215821 cites W2010647156 @default.
• W250215821 cites W2011239505 @default.
• W250215821 cites W2016752484 @default.
• W250215821 cites W2019683289 @default.
• W250215821 cites W2026617356 @default.
• W250215821 cites W2033497590 @default.
• W250215821 cites W2039212784 @default.
• W250215821 cites W2044874695 @default.
• W250215821 cites W2048567274 @default.
• W250215821 cites W2049727020 @default.
• W250215821 cites W2053095513 @default.
• W250215821 cites W2054530178 @default.
• W250215821 cites W2062493075 @default.
• W250215821 cites W2064192062 @default.
• W250215821 cites W2067696771 @default.
• W250215821 cites W2070910260 @default.
• W250215821 cites W2072181397 @default.
• W250215821 cites W2072799497 @default.
• W250215821 cites W2079747695 @default.
• W250215821 cites W2081920342 @default.
• W250215821 cites W2092340120 @default.
• W250215821 cites W2094395583 @default.
• W250215821 cites W2104314648 @default.
• W250215821 cites W2113593419 @default.
• W250215821 cites W2113991941 @default.
• W250215821 cites W2114570899 @default.
• W250215821 cites W2114825009 @default.
• W250215821 cites W2125306830 @default.
• W250215821 cites W2125747634 @default.
• W250215821 cites W2126162909 @default.
• W250215821 cites W2127192155 @default.
• W250215821 cites W2136550480 @default.
• W250215821 cites W2136667764 @default.
• W250215821 cites W2138226825 @default.
• W250215821 cites W2138416557 @default.
• W250215821 cites W2140449893 @default.
• W250215821 cites W2142504521 @default.
• W250215821 cites W2148755064 @default.
• W250215821 cites W2150551377 @default.
• W250215821 cites W2153980878 @default.
• W250215821 cites W2154024784 @default.
• W250215821 cites W2154285890 @default.
• W250215821 cites W2157057461 @default.
• W250215821 cites W2158373383 @default.
• W250215821 cites W2159664830 @default.
• W250215821 cites W2165985172 @default.
• W250215821 cites W2166304083 @default.
• W250215821 cites W2171243814 @default.
• W250215821 cites W4211173480 @default.
• W250215821 cites W4237590128 @default.
• W250215821 doi "https://doi.org/10.1371/journal.pone.0126009" @default.
• W250215821 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4429119" @default.
• W250215821 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25965910" @default.
• W250215821 hasPublicationYear "2015" @default.
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