FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2503965810> ?p ?o ?g. }

• W2503965810 endingPage "86" @default.
• W2503965810 startingPage "75" @default.
• W2503965810 abstract "Publisher Summary This chapter discusses the basic principles of temperature gradient gel electrophoresis (TGGE) and denaturing-gradient gel electrophoresis (DGGE). Both techniques are based on the principle that the electrophoretic mobility of double-stranded DNA fragments is significantly reduced by their partial denaturation. Because of the sequence dependence of the melting properties of DNA fragments, sequence variations are detected. TGGE/DGGE and related methods provide a very high sensitivity and are relatively easy and cheap to perform once the assays have been designed and optimized. The main advantages are the high detection rate and specificity and improved heterozygote detection. The methodology is simple, non-radioactive, and relatively non-toxic. Although the sensitivity of TGGE and DGGE in detecting point mutations in genetic disorders and other settings is reported to be close to 100 percent, these methods have never become popular, which is related to the perception that it is difficult to design adequate PCR primers and set up the assays. The disadvantages include the limitation of PCR fragment length to about 500 nucleotides, difficulties of analyzing GC-rich fragments, and the need for computer analysis of potential PCR fragments. However, once primers and conditions are chosen, TGGE/DGGE is a robust and easy to perform mutation screening method particularly well suited for the detection of known and unknown mutations in large genes, where high sensitivity is required and when large numbers of samples are to be tested." @default.
• W2503965810 created "2016-08-23" @default.
• W2503965810 creator A5073427658 @default.
• W2503965810 creator A5079008015 @default.
• W2503965810 date "2010-01-01" @default.
• W2503965810 modified "2023-09-30" @default.
• W2503965810 title "Temperature and Denaturing Gradient Gel Electrophoresis" @default.
• W2503965810 cites W114089393 @default.
• W2503965810 cites W1512997946 @default.
• W2503965810 cites W1528362997 @default.
• W2503965810 cites W1576596365 @default.
• W2503965810 cites W1598574080 @default.
• W2503965810 cites W1828930901 @default.
• W2503965810 cites W1913521901 @default.
• W2503965810 cites W1971328348 @default.
• W2503965810 cites W1973120450 @default.
• W2503965810 cites W1974512446 @default.
• W2503965810 cites W1975368227 @default.
• W2503965810 cites W1980180966 @default.
• W2503965810 cites W1985100700 @default.
• W2503965810 cites W1987412407 @default.
• W2503965810 cites W1990054281 @default.
• W2503965810 cites W1990896124 @default.
• W2503965810 cites W1992368452 @default.
• W2503965810 cites W1995950217 @default.
• W2503965810 cites W2004977228 @default.
• W2503965810 cites W2006294341 @default.
• W2503965810 cites W2013522623 @default.
• W2503965810 cites W2013912459 @default.
• W2503965810 cites W2016927124 @default.
• W2503965810 cites W2018249880 @default.
• W2503965810 cites W2018297119 @default.
• W2503965810 cites W2019878050 @default.
• W2503965810 cites W2039246283 @default.
• W2503965810 cites W2042368524 @default.
• W2503965810 cites W2047671235 @default.
• W2503965810 cites W2047851418 @default.
• W2503965810 cites W2049807819 @default.
• W2503965810 cites W2053631253 @default.
• W2503965810 cites W2054007006 @default.
• W2503965810 cites W2056533704 @default.
• W2503965810 cites W2057043036 @default.
• W2503965810 cites W2057582973 @default.
• W2503965810 cites W2058093686 @default.
• W2503965810 cites W2058179440 @default.
• W2503965810 cites W2061472576 @default.
• W2503965810 cites W2064112015 @default.
• W2503965810 cites W2064504648 @default.
• W2503965810 cites W2065185874 @default.
• W2503965810 cites W2066675000 @default.
• W2503965810 cites W2070484449 @default.
• W2503965810 cites W2070992140 @default.
• W2503965810 cites W2072163115 @default.
• W2503965810 cites W2072335033 @default.
• W2503965810 cites W2073694970 @default.
• W2503965810 cites W2074750435 @default.
• W2503965810 cites W2076012381 @default.
• W2503965810 cites W2076737381 @default.
• W2503965810 cites W2078886175 @default.
• W2503965810 cites W2085875229 @default.
• W2503965810 cites W2085879302 @default.
• W2503965810 cites W2100545413 @default.
• W2503965810 cites W2103170384 @default.
• W2503965810 cites W2111128847 @default.
• W2503965810 cites W2112384169 @default.
• W2503965810 cites W2115489730 @default.
• W2503965810 cites W2117130443 @default.
• W2503965810 cites W2123443649 @default.
• W2503965810 cites W2127570331 @default.
• W2503965810 cites W2128268820 @default.
• W2503965810 cites W2146129047 @default.
• W2503965810 cites W2147074998 @default.
• W2503965810 cites W2162100863 @default.
• W2503965810 cites W2162891006 @default.
• W2503965810 cites W2163379645 @default.
• W2503965810 cites W2164831804 @default.
• W2503965810 cites W2165440575 @default.
• W2503965810 cites W2167040524 @default.
• W2503965810 cites W2167403022 @default.
• W2503965810 cites W2323916371 @default.
• W2503965810 cites W2413531673 @default.
• W2503965810 doi "https://doi.org/10.1016/b978-0-12-374537-8.00006-7" @default.
• W2503965810 hasPublicationYear "2010" @default.
• W2503965810 type Work @default.
• W2503965810 sameAs 2503965810 @default.
• W2503965810 citedByCount "0" @default.
• W2503965810 crossrefType "book-chapter" @default.
• W2503965810 hasAuthorship W2503965810A5073427658 @default.
• W2503965810 hasAuthorship W2503965810A5079008015 @default.
• W2503965810 hasConcept C104317684 @default.
• W2503965810 hasConcept C185592680 @default.
• W2503965810 hasConcept C198549448 @default.
• W2503965810 hasConcept C40250595 @default.
• W2503965810 hasConcept C42062724 @default.
• W2503965810 hasConcept C43617362 @default.
• W2503965810 hasConcept C55493867 @default.
• W2503965810 hasConceptScore W2503965810C104317684 @default.
• W2503965810 hasConceptScore W2503965810C185592680 @default.

• next