FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2504320897> ?p ?o ?g. }

• W2504320897 endingPage "e0160702" @default.
• W2504320897 startingPage "e0160702" @default.
• W2504320897 abstract "We explore here the potential of a newly described technology, which is named PROFILER and is based on next generation sequencing of gene-specific lambda phage-displayed libraries, to rapidly and accurately map monoclonal antibody (mAb) epitopes. For this purpose, we used a novel mAb (designated 31E10/E7) directed against Neisserial Heparin-Binding Antigen (NHBA), a component of the anti-group B meningococcus Bexsero® vaccine. An NHBA phage-displayed library was affinity-selected with mAb 31E10/E7, followed by massive sequencing of the inserts present in antibody-selected phage pools. Insert analysis identified an amino acid stretch (D91-A128) in the N-terminal domain, which was shared by all of the mAb-enriched fragments. Moreover, a recombinant fragment encompassing this sequence could recapitulate the immunoreactivity of the entire NHBA molecule against mAb 31E10/E7. These results were confirmed using a panel of overlapping recombinant fragments derived from the NHBA vaccine variant and a set of chemically synthetized peptides covering the 10 most frequent antigenic variants. Furthermore, hydrogen-deuterium exchange mass-spectrometry analysis of the NHBA-mAb 31E10/E7 complex was also compatible with mapping of the epitope to the D91-A128 region. Collectively, these results indicate that the PROFILER technology can reliably identify epitope-containing antigenic fragments and requires considerably less work, time and reagents than other epitope mapping methods." @default.
• W2504320897 created "2016-08-23" @default.
• W2504320897 creator A5003469840 @default.
• W2504320897 creator A5003928174 @default.
• W2504320897 creator A5011354377 @default.
• W2504320897 creator A5016874972 @default.
• W2504320897 creator A5017379911 @default.
• W2504320897 creator A5018624822 @default.
• W2504320897 creator A5023270760 @default.
• W2504320897 creator A5030802364 @default.
• W2504320897 creator A5038117660 @default.
• W2504320897 creator A5038560996 @default.
• W2504320897 creator A5046613129 @default.
• W2504320897 creator A5052800632 @default.
• W2504320897 creator A5064207717 @default.
• W2504320897 creator A5066558058 @default.
• W2504320897 creator A5074741886 @default.
• W2504320897 creator A5086735063 @default.
• W2504320897 creator A5086992506 @default.
• W2504320897 creator A5089959950 @default.
• W2504320897 creator A5091209766 @default.
• W2504320897 date "2016-08-10" @default.
• W2504320897 modified "2023-10-16" @default.
• W2504320897 title "Epitope Mapping of a Monoclonal Antibody Directed against Neisserial Heparin Binding Antigen Using Next Generation Sequencing of Antigen-Specific Libraries" @default.
• W2504320897 cites W129165853 @default.
• W2504320897 cites W1597163568 @default.
• W2504320897 cites W1656961899 @default.
• W2504320897 cites W1965030023 @default.
• W2504320897 cites W1965487319 @default.
• W2504320897 cites W1970649974 @default.
• W2504320897 cites W1976801077 @default.
• W2504320897 cites W1983416604 @default.
• W2504320897 cites W1993074788 @default.
• W2504320897 cites W1997902072 @default.
• W2504320897 cites W2011114953 @default.
• W2504320897 cites W2012763858 @default.
• W2504320897 cites W2018794676 @default.
• W2504320897 cites W2036301066 @default.
• W2504320897 cites W2042492527 @default.
• W2504320897 cites W2048023847 @default.
• W2504320897 cites W2052152476 @default.
• W2504320897 cites W2063607410 @default.
• W2504320897 cites W2088953839 @default.
• W2504320897 cites W2094573352 @default.
• W2504320897 cites W2136195832 @default.
• W2504320897 cites W2148404862 @default.
• W2504320897 cites W2162153165 @default.
• W2504320897 cites W2167963851 @default.
• W2504320897 cites W2196235146 @default.
• W2504320897 cites W2198744375 @default.
• W2504320897 cites W2288527324 @default.
• W2504320897 cites W2295267749 @default.
• W2504320897 cites W4249066710 @default.
• W2504320897 doi "https://doi.org/10.1371/journal.pone.0160702" @default.
• W2504320897 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4980009" @default.
• W2504320897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27508302" @default.
• W2504320897 hasPublicationYear "2016" @default.
• W2504320897 type Work @default.
• W2504320897 sameAs 2504320897 @default.
• W2504320897 citedByCount "10" @default.
• W2504320897 countsByYear W25043208972018 @default.
• W2504320897 countsByYear W25043208972019 @default.
• W2504320897 countsByYear W25043208972020 @default.
• W2504320897 countsByYear W25043208972021 @default.
• W2504320897 countsByYear W25043208972023 @default.
• W2504320897 crossrefType "journal-article" @default.
• W2504320897 hasAuthorship W2504320897A5003469840 @default.
• W2504320897 hasAuthorship W2504320897A5003928174 @default.
• W2504320897 hasAuthorship W2504320897A5011354377 @default.
• W2504320897 hasAuthorship W2504320897A5016874972 @default.
• W2504320897 hasAuthorship W2504320897A5017379911 @default.
• W2504320897 hasAuthorship W2504320897A5018624822 @default.
• W2504320897 hasAuthorship W2504320897A5023270760 @default.
• W2504320897 hasAuthorship W2504320897A5030802364 @default.
• W2504320897 hasAuthorship W2504320897A5038117660 @default.
• W2504320897 hasAuthorship W2504320897A5038560996 @default.
• W2504320897 hasAuthorship W2504320897A5046613129 @default.
• W2504320897 hasAuthorship W2504320897A5052800632 @default.
• W2504320897 hasAuthorship W2504320897A5064207717 @default.
• W2504320897 hasAuthorship W2504320897A5066558058 @default.
• W2504320897 hasAuthorship W2504320897A5074741886 @default.
• W2504320897 hasAuthorship W2504320897A5086735063 @default.
• W2504320897 hasAuthorship W2504320897A5086992506 @default.
• W2504320897 hasAuthorship W2504320897A5089959950 @default.
• W2504320897 hasAuthorship W2504320897A5091209766 @default.
• W2504320897 hasBestOaLocation W25043208971 @default.
• W2504320897 hasConcept C104317684 @default.
• W2504320897 hasConcept C147483822 @default.
• W2504320897 hasConcept C153911025 @default.
• W2504320897 hasConcept C159047783 @default.
• W2504320897 hasConcept C159654299 @default.
• W2504320897 hasConcept C167625842 @default.
• W2504320897 hasConcept C185592680 @default.
• W2504320897 hasConcept C195616568 @default.
• W2504320897 hasConcept C2776471321 @default.
• W2504320897 hasConcept C2781241564 @default.
• W2504320897 hasConcept C40767141 @default.
• W2504320897 hasConcept C542903549 @default.

• next