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- W2505254073 abstract "Evaluation of pollen viability and pollen size variation is instrumental in polycross testing. Polymixes used for polycross should be adjusted so that they are composed of equal numbers of viable pollen grains from each male. In this study, yearly and clonal variation in jack pine (Pinus banksiana LAMB.) pollen size is evaluated for pollen collected from 15 clones and 25 clones in 1992 and 1993, respectively; 13 clones were common to both years. In vitro pollen viability was assessed by using agar with 5% sucrose. Pollen sizing was achieved with a hemocytometer, commonly used by medical technologists to count various blood cells. Pollen viability averaged 70%, 86%, and 90% for 1992 freshly collected pollen, 1992 pollen stored for 1 year, and 1993 freshly collected pollen, respectively. Clonal viability varied between 54% and 90%, 67% and 95%, and 75% and 98% for 1992 freshly collected pollen, 1992 pollen stored for 1 year, and 1993 freshly collected pollen, respectively. The number of pollen grains per mg (MG) or ml (ML) of dry pollen was quite variable between years and among clones. ML averaged 16.4 million and 17.7 million in 1992 and 1993, respectively. The highest clonal ML value in 1992 and 1993 was 1.8 times and 1.7 times that of the smallest value, respectively. No pollen morphometric measure was valid in estimating reliably ML or MG. Adjustments to obtain equal numbers of viable pollen grains for each male parent in a polymix are recommended." @default.
- W2505254073 created "2016-08-23" @default.
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- W2505254073 date "1995-10-02" @default.
- W2505254073 modified "2023-09-23" @default.
- W2505254073 title "Pollen sizing in Jack pine (Pinus banksiana Lamb.) with a hemocytometer" @default.
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