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• W2506234310 endingPage "gkw690" @default.
• W2506234310 startingPage "gkw690" @default.
• W2506234310 abstract "Helicases couple ATP hydrolysis to nucleic acid binding and unwinding via molecular mechanisms that remain poorly defined for most enzyme subfamilies within the superfamily 2 (SF2) helicase group. A crystal structure of the PriA SF2 DNA helicase, which governs restart of prematurely terminated replication processes in bacteria, revealed the presence of an aromatic-rich loop (ARL) on the presumptive DNA-binding surface of the enzyme. The position and sequence of the ARL was similar to loops known to couple ATP hydrolysis with DNA binding in a subset of other SF2 enzymes, however, the roles of the ARL in PriA had not been investigated. Here, we show that changes within the ARL sequence uncouple PriA ATPase activity from DNA binding. In vitro protein-DNA crosslinking experiments define a residue- and nucleotide-specific interaction map for PriA, showing that the ARL binds replication fork junctions whereas other sites bind the leading or lagging strands. We propose that DNA binding to the ARL allosterically triggers ATP hydrolysis in PriA. Additional SF2 helicases with similarly positioned loops may also couple DNA binding to ATP hydrolysis using related mechanisms." @default.
• W2506234310 created "2016-08-23" @default.
• W2506234310 creator A5050253186 @default.
• W2506234310 creator A5075210236 @default.
• W2506234310 date "2016-08-02" @default.
• W2506234310 modified "2023-09-27" @default.
• W2506234310 title "An aromatic-rich loop couples DNA binding and ATP hydrolysis in the PriA DNA helicase" @default.
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• W2506234310 cites W1985662030 @default.
• W2506234310 cites W1987314616 @default.
• W2506234310 cites W1987914894 @default.
• W2506234310 cites W1988289713 @default.
• W2506234310 cites W1992657957 @default.
• W2506234310 cites W1996555720 @default.
• W2506234310 cites W1996984611 @default.
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• W2506234310 cites W2000516676 @default.
• W2506234310 cites W2008250450 @default.
• W2506234310 cites W2009021135 @default.
• W2506234310 cites W2013435843 @default.
• W2506234310 cites W2018325248 @default.
• W2506234310 cites W2021560988 @default.
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• W2506234310 cites W2026133647 @default.
• W2506234310 cites W2026957636 @default.
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• W2506234310 cites W2028941521 @default.
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• W2506234310 cites W2035595761 @default.
• W2506234310 cites W2037978116 @default.
• W2506234310 cites W2045941135 @default.
• W2506234310 cites W2054129096 @default.
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• W2506234310 cites W2089298763 @default.
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• W2506234310 doi "https://doi.org/10.1093/nar/gkw690" @default.
• W2506234310 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5175346" @default.
• W2506234310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27484483" @default.
• W2506234310 hasPublicationYear "2016" @default.
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