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- W2507918686 abstract "Viral quasispecies sequences reconstruction by analyzing HTS/Next Generation Sequencing (NGS) data will contribute to understanding the roles and interactions of infected animal and viral variants for improving animal health and productivity. This understanding is necessary for animal health, and production efficiency, which is one of the goals included in Blueprint for USDA Efforts in Agricultural Animal Genomics. Cloning and sequencing have been used to show that infectious bronchitis virus (IBV) variants exist in infected poultry as well as in several widely used commercial live attenuated vaccines. To validate the reconstruction of the quasispecies using different methods, one computed the pairwise distances between Sanger clones and reconstructed variants. Average Distance to Clones (ADC) and Average Prediction Error (APE) are analogous to sensitivity, respectively. The results suggest that using different methods with different parameter calibration and parameter settings can improve the solution and its predictive power for the quasispecies inference problem in terms of recall and precision." @default.
- W2507918686 created "2016-09-16" @default.
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- W2507918686 date "2016-08-26" @default.
- W2507918686 modified "2023-10-17" @default.
- W2507918686 title "Reconstruction of Infectious Bronchitis Virus Quasispecies from NGS Data" @default.
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- W2507918686 doi "https://doi.org/10.1002/9781119272182.ch17" @default.
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