FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2507949351> ?p ?o ?g. }

• W2507949351 abstract "A GC-MS method is reported for the quantitative analysis of S-nitrosothiols (RSNO) derived from endogenous low- and high-molecular mass thiols (RSH) including hemoglobin, cysteine, glutathione, N-acetylcysteine, and the exogenous N-acetylcysteine ethyl ester. The method is based on the conversion of RSNO to nitrite by aqueous Na2S (S2-). 15N-Labelled analogs (RS15NO) or 15N-labelled nitrite and nitrate were used as internal standards. The nitrite (14NO2- and 15NO2-) and nitrate (O14NO2- and O15NO2- anions were derivatised by pentafluorobenzyl (PFB) bromide (PFB-Br) in aqueous acetone and their PFB derivatives were separated by gas chromatography. After electron-capture negative-ion chemical ionization, the anions were separated by mass spectrometry and detected by selected-ion monitoring of m/z 46 for 14NO2-, m/z 47 for 15NO2-, m/z 62 for O14NO2-, and m/z 63 for O15NO2-. The expected thionitrites (-S14NO and -S15NO) were not detected, suggesting that they are intermediates and rapidly exchange their S by O from water, presumably prior to PFB-Br derivatization. The reaction of S2- with RSNO and sodium nitroprusside (SNP) resulted in the formation of nitrite and nitrate as the major and minor reaction products, respectively. The novel Na2S procedure was compared with established procedures based on the use of aqueous HgCl2 or cysteine/Cu2+ reagents to convert the S-nitroso group to nitrite. Our results provide evidence for an equilibrium S-transnitrosylation reaction between S2- with RSNO in buffered solutions of neutral pH. Use of Na2S in molar excess over RSNO shifts this reaction to the right, thus allowing almost complete conversion of RSNO to nitrite and nitrate. The Na2S procedure should be useful for the quantitative determination of RSNO as nitrite and nitrate after PFB-Br derivatization and GC-MS analysis. The Na2S procedure may also contribute to explore the complex reactions of S2- with RSNO, SNP and other NO-containing compounds." @default.
• W2507949351 created "2016-09-16" @default.
• W2507949351 creator A5007374293 @default.
• W2507949351 creator A5017241014 @default.
• W2507949351 creator A5044012246 @default.
• W2507949351 creator A5045030779 @default.
• W2507949351 date "2017-02-01" @default.
• W2507949351 modified "2023-09-24" @default.
• W2507949351 title "GC-ECNICI-MS analysis of S-nitrosothiols and nitroprusside after treatment with aqueous sulphide (S2−) and derivatization with pentafluorobenzyl bromide: Evidence of S-transnitrosylation and formation of nitrite and nitrate" @default.
• W2507949351 cites W1553766496 @default.
• W2507949351 cites W1570790098 @default.
• W2507949351 cites W1869646924 @default.
• W2507949351 cites W187114146 @default.
• W2507949351 cites W1972890330 @default.
• W2507949351 cites W1994703917 @default.
• W2507949351 cites W2002192155 @default.
• W2507949351 cites W2004160418 @default.
• W2507949351 cites W2011177814 @default.
• W2507949351 cites W2012695220 @default.
• W2507949351 cites W2017632468 @default.
• W2507949351 cites W2021707617 @default.
• W2507949351 cites W2029337791 @default.
• W2507949351 cites W2036187656 @default.
• W2507949351 cites W2037151440 @default.
• W2507949351 cites W2045846824 @default.
• W2507949351 cites W2056378139 @default.
• W2507949351 cites W2059441563 @default.
• W2507949351 cites W2061039308 @default.
• W2507949351 cites W2064549275 @default.
• W2507949351 cites W2065085951 @default.
• W2507949351 cites W2073538982 @default.
• W2507949351 cites W2076291255 @default.
• W2507949351 cites W2079148006 @default.
• W2507949351 cites W2091793758 @default.
• W2507949351 cites W2093742700 @default.
• W2507949351 cites W2106312949 @default.
• W2507949351 cites W2111363155 @default.
• W2507949351 cites W2181235140 @default.
• W2507949351 cites W2283112134 @default.
• W2507949351 cites W2284168863 @default.
• W2507949351 cites W6722647 @default.
• W2507949351 doi "https://doi.org/10.1016/j.jchromb.2016.09.002" @default.
• W2507949351 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27614419" @default.
• W2507949351 hasPublicationYear "2017" @default.
• W2507949351 type Work @default.
• W2507949351 sameAs 2507949351 @default.
• W2507949351 citedByCount "1" @default.
• W2507949351 countsByYear W25079493512019 @default.
• W2507949351 crossrefType "journal-article" @default.
• W2507949351 hasAuthorship W2507949351A5007374293 @default.
• W2507949351 hasAuthorship W2507949351A5017241014 @default.
• W2507949351 hasAuthorship W2507949351A5044012246 @default.
• W2507949351 hasAuthorship W2507949351A5045030779 @default.
• W2507949351 hasConcept C145148216 @default.
• W2507949351 hasConcept C162356407 @default.
• W2507949351 hasConcept C178790620 @default.
• W2507949351 hasConcept C179104552 @default.
• W2507949351 hasConcept C184651966 @default.
• W2507949351 hasConcept C185592680 @default.
• W2507949351 hasConcept C198291218 @default.
• W2507949351 hasConcept C2776179834 @default.
• W2507949351 hasConcept C2776300020 @default.
• W2507949351 hasConcept C2776384668 @default.
• W2507949351 hasConcept C2776923573 @default.
• W2507949351 hasConcept C2777633056 @default.
• W2507949351 hasConcept C40875361 @default.
• W2507949351 hasConcept C43617362 @default.
• W2507949351 hasConcept C51884965 @default.
• W2507949351 hasConceptScore W2507949351C145148216 @default.
• W2507949351 hasConceptScore W2507949351C162356407 @default.
• W2507949351 hasConceptScore W2507949351C178790620 @default.
• W2507949351 hasConceptScore W2507949351C179104552 @default.
• W2507949351 hasConceptScore W2507949351C184651966 @default.
• W2507949351 hasConceptScore W2507949351C185592680 @default.
• W2507949351 hasConceptScore W2507949351C198291218 @default.
• W2507949351 hasConceptScore W2507949351C2776179834 @default.
• W2507949351 hasConceptScore W2507949351C2776300020 @default.
• W2507949351 hasConceptScore W2507949351C2776384668 @default.
• W2507949351 hasConceptScore W2507949351C2776923573 @default.
• W2507949351 hasConceptScore W2507949351C2777633056 @default.
• W2507949351 hasConceptScore W2507949351C40875361 @default.
• W2507949351 hasConceptScore W2507949351C43617362 @default.
• W2507949351 hasConceptScore W2507949351C51884965 @default.
• W2507949351 hasFunder F4320320879 @default.
• W2507949351 hasLocation W25079493511 @default.
• W2507949351 hasLocation W25079493512 @default.
• W2507949351 hasOpenAccess W2507949351 @default.
• W2507949351 hasPrimaryLocation W25079493511 @default.
• W2507949351 hasRelatedWork W1543207566 @default.
• W2507949351 hasRelatedWork W1579842972 @default.
• W2507949351 hasRelatedWork W1612987616 @default.
• W2507949351 hasRelatedWork W1965026439 @default.
• W2507949351 hasRelatedWork W1966985035 @default.
• W2507949351 hasRelatedWork W1974992905 @default.
• W2507949351 hasRelatedWork W1986709512 @default.
• W2507949351 hasRelatedWork W2003310512 @default.
• W2507949351 hasRelatedWork W2006278917 @default.
• W2507949351 hasRelatedWork W2007190913 @default.
• W2507949351 hasRelatedWork W2046529200 @default.
• W2507949351 hasRelatedWork W2047466663 @default.

• next