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• W2507966312 abstract "Recent advances in the ease with which the genomes of small circular single-stranded DNA viruses can be amplified, cloned, and sequenced have greatly acceler- ated the rate at which full genome sequences of mastrevi- ruses (family Geminiviridae, genus Mastrevirus) are being deposited in public sequence databases. Although guide- lines currently exist for species-level classification of newly determined, complete mastrevirus genome sequen- ces, these are difficult to apply to large sequence datasets and are permissive enough that, effectively, a high degree of leeway exists for the proposal of new species and strains. The lack of a standardised and rigorous method for testing whether a new genome sequence deserves such a classifi- cation is resulting in increasing numbers of questionable mastrevirus species proposals. Importantly, the recom- mended sequence alignment and pairwise identity calcu- lation protocols of the current guidelines could easily be modified to make the classification of newly determined mastrevirus genome sequences significantly more objec- tive. Here, we propose modified versions of these protocols that should substantially minimise the degree of classifi- cation inconsistency that is permissible under the current system. To facilitate the objective application of these guidelines for mastrevirus species demarcation, we addi- tionally present a user-friendly computer program, SDT (species demarcation tool), for calculating and graphically" @default.
• W2507966312 created "2016-09-16" @default.
• W2507966312 creator A5079936146 @default.
• W2507966312 date "2013-01-01" @default.
• W2507966312 modified "2023-09-23" @default.
• W2507966312 title "A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae)" @default.
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